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Overview
A step-by-step tour through the complete process of doing proteomics. With easy-to-follow instructions, complete with many helpful hints and explanations, leading investigators and pioneers in the field show how to make protein extracts, reproducibly run them on 2-D gels, detect them, analyze the data, and precisely identify each protein. The book covers the latest methods of using carrier ampholytes in the 1st dimension, casting and running immobilized pH gradient 2-D gels, MALDI-TOF-based peptide mapping, automated tandem mass spectrometry, and nanoelectrospray ionization technology. For the 2nd dimension, there are methods for running flatbed or vertical gels and for protein detection using autoradiography, and Coomassie, silver, and reversible metal-chelate stains. 2-D Proteome Analysis Prools is the most complete guide for using proteomics to answer biological questions.
The book contains black-and-white illustrations.
Editorials
Alvin Telser
This book is a detailed compilation of the theory and practice of analyzing total cellular protein by two-dimensional gel electrophoresis. According to the editor, it is intended as a benchtop manual for anyone who uses these techniques. With the number of sophisticated techniques now in use in many areas of life sciences research, this will be a valuable volume and it fully meets the editor's stated objectives. It is written for beginners and for more experienced researchers who may wish to expand on or improve their use of the 2-D gel electrophoretic techniques. The 55 chapters are written by over 100 contributors, all of whom are quite expert in their specialty. This book contains a detailed description of the techniques (reagents, equipment, and trouble-shooting) that are widely used for the analysis of total cellular proteins. The illustrations are very helpful and easy to understand. Several of the chapters explain how the techniques can be standardized so valid comparisons of results can be made from laboratory to laboratory. This is a very worthwhile guide to the technique of two-dimensional gel electrophoresis. It would be a valuable addition to a lab carrying out these procedures and would make a very useful addition to any life science library.Booknews
Comprises 55 contributions that are intended as a guide both for novices to 2-D gels and for experienced practitioners who want to try the newer techniques. The contributions guide researchers through the complete process of working with 2-D protein gels from making the protein extract to finally identifying the proteins of interest. The volume includes protocols for generating 2-D protein extracts from most of the standard model organisms, including bacteria, yeasts, nematodes, plants, mice, and humans. Each chapter includes a description of the protocol, the basic theory behind it, a list of the equipment and reagents necessary for the protocol, a step-by-step guide to the experiment, and hints for dealing with problems. Annotation c. by Book News, Inc., Portland, Or.From The Critics
Reviewer: Alvin Telser, PhD(Northwestern University Feinberg School of Medicine)Description: This book is a detailed compilation of the theory and practice of analyzing total cellular protein by two-dimensional gel electrophoresis.
Purpose: According to the editor, it is intended as a benchtop manual for anyone who uses these techniques. With the number of sophisticated techniques now in use in many areas of life sciences research, this will be a valuable volume and it fully meets the editor's stated objectives.
Audience: It is written for beginners and for more experienced researchers who may wish to expand on or improve their use of the 2-D gel electrophoretic techniques. The 55 chapters are written by over 100 contributors, all of whom are quite expert in their specialty.
Features: This book contains a detailed description of the techniques (reagents, equipment, and trouble-shooting) that are widely used for the analysis of total cellular proteins. The illustrations are very helpful and easy to understand. Several of the chapters explain how the techniques can be standardized so valid comparisons of results can be made from laboratory to laboratory.
Assessment: This is a very worthwhile guide to the technique of two-dimensional gel electrophoresis. It would be a valuable addition to a lab carrying out these procedures and would make a very useful addition to any life science library.
5 Stars! from Doody