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Overview
Basic Protein and Peptide Prools offers an excellent collection of reproducible, step-by-step laboratory methods covering three major areas: (1) the quantitation and characterization of proteins, (2) the electrophoretic and blotting procedures used in protein isolation and characterization, and (3) the analysis of protein and peptide structure. THOUSANDS of labs are already using Basic Protein and Peptide Prools-you should be too!
The book contains black-and-white illustrations.
Synopsis
Basic Protein and Peptide Protocols offers an excellent collection of reproducible, step-by-step laboratory methods covering three major areas: (1) the quantitation and characterization of proteins, (2) the electrophoretic and blotting procedures used in protein isolation and characterization, and (3) the analysis of protein and peptide structure. THOUSANDS of labs are already using Basic Protein and Peptide Protocols-you should be too!
Ada A. Cole
This book is written to update an earlier volume in the Methods in Molecular Biology series and is aimed at researchers who have become involved in the biochemical analyses of proteins and peptides. The text contains both newly developed protocols as well as revised established procedures using new technologies. The analytical methods included here reflect newer technologies such as capillary electrophoresis, immobilized pH gradients in isoelectric focusing, immunogold reagents and the BCA assay for proteins. Also included are newer applications that improve sensitivities, resolution, speed, or ease of use, including chemiluminescent systems and lectins. Each protocol provides an introduction, list of materials needed, step-by-step methods, notes, and references. This book is written as a bench top manual and guide for those new to protein biochemistry. A researcher not familiar with the procedure should be able to follow the directions without difficulty. The figures that are included clearly illustrate the types of results and sensitivities to be expected with the protocols. The references listed cover the development of the technique as published in the literature. The table of contents and index are adequate. This book will serve as an excellent guidebook for researchers who are trained as molecular biologists and have become interested in the analytical methods of biochemistry. Each chapter lists advantages and disadvantages including sensitivity and limitations. This is an excellent bench top edition.
Editorials
From The Critics
Reviewer: Ada A. Cole, PhD(Rush Medical College of Rush University)Description: This book is written to update an earlier volume in the Methods in Molecular Biology series and is aimed at researchers who have become involved in the biochemical analyses of proteins and peptides. The text contains both newly developed protocols as well as revised established procedures using new technologies.
Purpose: The analytical methods included here reflect newer technologies such as capillary electrophoresis, immobilized pH gradients in isoelectric focusing, immunogold reagents and the BCA assay for proteins. Also included are newer applications that improve sensitivities, resolution, speed, or ease of use, including chemiluminescent systems and lectins. Each protocol provides an introduction, list of materials needed, step-by-step methods, notes, and references.
Audience: This book is written as a bench top manual and guide for those new to protein biochemistry. A researcher not familiar with the procedure should be able to follow the directions without difficulty.
Features: The figures that are included clearly illustrate the types of results and sensitivities to be expected with the protocols. The references listed cover the development of the technique as published in the literature. The table of contents and index are adequate.
Assessment: This book will serve as an excellent guidebook for researchers who are trained as molecular biologists and have become interested in the analytical methods of biochemistry. Each chapter lists advantages and disadvantages including sensitivity and limitations. This is an excellent bench top edition.
Ada A. Cole
This book is written to update an earlier volume in the Methods in Molecular Biology series and is aimed at researchers who have become involved in the biochemical analyses of proteins and peptides. The text contains both newly developed protocols as well as revised established procedures using new technologies. The analytical methods included here reflect newer technologies such as capillary electrophoresis, immobilized pH gradients in isoelectric focusing, immunogold reagents and the BCA assay for proteins. Also included are newer applications that improve sensitivities, resolution, speed, or ease of use, including chemiluminescent systems and lectins. Each protocol provides an introduction, list of materials needed, step-by-step methods, notes, and references. This book is written as a bench top manual and guide for those new to protein biochemistry. A researcher not familiar with the procedure should be able to follow the directions without difficulty. The figures that are included clearly illustrate the types of results and sensitivities to be expected with the protocols. The references listed cover the development of the technique as published in the literature. The table of contents and index are adequate. This book will serve as an excellent guidebook for researchers who are trained as molecular biologists and have become interested in the analytical methods of biochemistry. Each chapter lists advantages and disadvantages including sensitivity and limitations. This is an excellent bench top edition.Booknews
An update to Walker's classic Proteins (1984), adding many new tested basic laboratory methods for protein and peptide analysis, for both those new to the protein chemistry laboratory and more established workers who wish to try a new technique for the first time. Among the key protocols included are protein and peptide electrophoresis techniques, gel staining methods, methods for protein quantitation, peptide mapping, protein blotting and various detection systems, peptide sequencing, glycoprotein analysis, production of polyclonal and monoclonal antibodies, ELISA, the use of immobilized pH gradients in isoelectric focusing, IgG purification, the use of immunogold reagents, and the detection and analysis of thiol groups. Plastic comb binding. Annotation c. Book News, Inc., Portland, OR (booknews.com)3 Stars from Doody