Immunochemistry 1: A Practical Approach, Vol. 177

Immunochemistry is of immense importance to virtually all areas of modern biology and medicine. Immunochemistry I and its companion volume, Immunochemistry II, offer a comprehensive compendium of the latest techniques in immunochemistry in a clear and accessible format. Volume I details the current strategies and methods used in the production of high quality antibodies, including chemically and genetically engineered antibodies, human monoclonal antibodies from B lymphocytes, anti-tumor antibodies, and antibodies packaged in eggs. Other topics include the uses of enzymes and antibodies in immunoassays; the standardization of antigens and antibodies; the latest ELISA techniques including enzyme amplification systems; enzyme immunoassays for detecting cell-surface molecules, single cell products, and proliferation; photoluminescence immunoassays; the incorporation of antibodies and their fragments into immunotoxins; and the use of synthetic peptides in mapping the epitopes of both antibodies and T-cells.
Volume II discusses the measurements of immunologically relevant molecules; immunofluorescence and immunoenzyme histochemistry; the use of synthetic peptides and liposomes in immunology; the use of antibodies as analytical probes and separation tools in fluorescence, blotting, and affinity chromatography; the measurement of antibody affinity; solid-phase assays of avidity; confocal laser scanning microscopy; flow cytofluorimetry; analysis of adhesion molecules; assays for complement receptors and for complement components. Each chapter includes clear technical advice, hints and tips, and sample results to help insure practical success.

The book contains black-and-white illustrations.

Immunochemistry is of immense importance to virtually all areas of modern biology and medicine. Immunochemistry I and its companion volume, Immunochemistry II, offer a comprehensive compendium of the latest techniques in immunochemistry in a clear and accessible format. Volume I details the current strategies and methods used in the production of high quality antibodies, including chemically and genetically engineered antibodies, human monoclonal antibodies from B lymphocytes, anti-tumor antibodies, and antibodies packaged in eggs. Other topics include the uses of enzymes and antibodies in immunoassays; the standardization of antigens and antibodies; the latest ELISA techniques including enzyme amplification systems; enzyme immunoassays for detecting cell-surface molecules, single cell products, and proliferation; photoluminescence immunoassays; the incorporation of antibodies and their fragments into immunotoxins; and the use of synthetic peptides in mapping the epitopes of both antibodies and T-cells.
Volume II discusses the measurements of immunologically relevant molecules; immunofluorescence and immunoenzyme histochemistry; the use of synthetic peptides and liposomes in immunology; the use of antibodies as analytical probes and separation tools in fluorescence, blotting, and affinity chromatography; the measurement of antibody affinity; solid-phase assays of avidity; confocal laser scanning microscopy; flow cytofluorimetry; analysis of adhesion molecules; assays for complement receptors and for complement components. Each chapter includes clear technical advice, hints and tips, and sample results to help insure practical success.

John A. Robinson

This is a two-volume set edited by two experts in immunochemistry and written by a diverse set of comparably expert contributors that describe their immunochemical and immunoserological techniques in an outstanding ""how-to"" manual. The books contain step-by-step boxed protocols buttressed by sufficient theory and applications (both current and future) to elevate it above cookbook status. It will be of immediate and almost daily help to graduate students, post doctoral students, and technicians. The confocal microscopy chapter has spectacular color micrographs that convey the power of this imaging technique. Most of the other chapters, although not as dramatic, are well written, concise, and practical as advertised in the title. This book is highly recommended to virtually all scientists toiling in the immunochemical vineyard.

Johnstone, Alan P. (St George's Hospital Medical School, London); Turner, Malcolm W. (Institute of Child Health, London)

The contributors represent the specialties of immunobiology, general surgery, pharmacology, and pathology. Most are from universities in the U.K., the Netherlands, and the U.S., as well as research institutes in Australia and Denmark. Institutions prominently represented include Chiron Institute, Statens Serum Institut, Maastricht Univ, Univ of Texas Southwestern Medical Center, and Univ of East London.