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Overview
Experts present methods and prools essential for understanding parasites at the molecular level. The prools cover culturing techniques for the major experimental organisms, methods for isolating and processing nucleic acids and proteins, PCR-based prools for parasite identification, gene isolation and mutation, antibody-based procedures, chromosome and epitope mapping, flow cytometry, RNA sequencing, and parasite transformation.
Synopsis
Experts present methods and protocols essential for understanding parasites at the molecular level. The protocols cover culturing techniques for the major experimental organisms, methods for isolating and processing nucleic acids and proteins, PCR-based protocols for parasite identification, gene isolation and mutation, antibody-based procedures, chromosome and epitope mapping, flow cytometry, RNA sequencing, and parasite transformation.
Booknews
The study of parasitic organisms--particularly those responsible for major diseases such as malaria, schistosomiasis, and trypanosomiasis--has been propelled by advances in recombinant DNA technology and, over the last decade, considerable progress has been made in understanding these organisms at the molecular level. This volume presents a collection of 34 readily reproducible protocols describing techniques proven to be of major importance in the emerging field of molecular parasitology. Chapters are contributed by leading figures who have either developed, or are especially familiar with, each technique. Plastic comb binding. Annotation c. Book News, Inc., Portland, OR (booknews.com)