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Overview
This greatly enhanced second edition introduces 60 critically important new chapters dealing with topics and techniques that have emerged since the first edition, as well as significantly updating the remaining articles. The new chapters cover many rapidly developing areas, particularly the application of mass spectrometry in protein characterization, as well as the now well-established 2-D PAGE technique in proteomics. The section on glycoprotein analysis has also been significantly expanded, and methods for the production of single-chain and phage-displayed antibodies have been added to the section on antibodies. Each readily reproducible method follows the highly praised format of the Methods in Molecular Biology™ series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls and on modifying the method to function within your own experimental circumstances.The book contains black-and-white illustrations.
Synopsis
Since the publication of the bestselling second edition of John Walker’s widely acclaimed Protein Protocols Handbook, there have been continual methodological developments in the field of protein chemistry. This greatly enhanced third edition introduces 57 critically important new chapters, as well as significantly updating the previous edition's tried-and-true methods. Although the timely new chapters are spread throughout all of the book, the vital section on post-translational modifications has been expanded most to reflect the increasing importance of these modifications in the understanding of protein function.
Each readily reproducible method follows the highly praised format of the Methods in Molecular Biology™ series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls, or on modifying the method to function within your own experimental circumstances. The expert authors of each chapter have demonstrated a hands-on mastery of the methods described, fine-tuned here for optimal productivity.
Comprehensive, cutting-edge, and highly practical, The Protein Protocols Handbook, Third Edition is today's indispensable benchtop manual and guide, not only for all those new to the protein chemistry laboratory, but also for those established workers seeking to broaden their armamentarium of techniques in the urgent search for rapid and robust results
Doody Review Services
Reviewer:Eugene A Davidson, PhD(Georgetown University School of Medicine)
Description:This is a compilation of methods used in protein analysis and characterization. The 164 contributions cover almost all aspects of protein identification. This second edition (first edition published in 1996) contains much new material, especially in areas relating to proteomics.
Purpose:The book is intended as a laboratory companion for investigators working with proteins. The intent is to provide protocols with sufficient detail so as to allow their ready adaptation in most working laboratories. These are useful objectives and generally well met.
Audience:It is targeted to graduate students, fellows, and senior investigators -- in short, those in the laboratory environment. The multiauthor list includes very well known authorities with worldwide representation. The editor has done a fine job in assembling this group.
Features:A book that intends to provide complete protocol coverage for scientists working with proteins must, of necessity, be large. This contribution is no exception and might have profited from more critical editing. For example, it is not essential to detail six general methods for estimating protein concentration when no more than two of these are currently in routine use. Nevertheless, the breadth of techniques covered is large and this alone makes the book of practical value. There are 164 chapters in all, divided into seven major sections. In addition to quantitation, areas covered include electrophoretic methods, blotting, chemical modification, glycoproteins and antibody methods (with a separate section on monoclonal antibodies). Each of the chapters has a detailed protocol for the given procedure; bibliographic citations range from negligible to adequate. The protocols are provided with sufficient detail so as to allow implementation as new methods in any working laboratory. In most cases, a brief discussion of the theory behind the method provides useful material, especially for students. In addition, pitfalls and limitations are usually indicated, an important asset to any collection of protocols. It can easily be envisioned that this book would become the subject of a tug of war in a busy laboratory. Most investigators will find value here as will students working in almost any area dealing with proteins.
Assessment:This is a compilation not found in other single sources. The very broad scope has considerable practical value. I personally want this in my laboratory.
Editorials
Booknews
Contains 144 analytical techniques for studying proteins and peptides (updated from the author's Basic Protein and Peptide Protocols, and including 100 new ones). Instructions are step-by-step and include troubleshooting tips, alternative procedures, explanations, and comprehensive lists of reagents and suppliers. Topics covered range from protein and peptide electrophoresis, detection in gels, and blotting methods, to protein and peptide characterization, detection of glycoproteins, immunochemical techniques, and monoclonal antibodies. For both novice and experienced investigators. Plastic comb binding. Annotation c. Book News, Inc., Portland, OR (booknews.com)From The Critics
This second edition of a collection of 164 reproducible protocols introduces topics and techniques that have emerged in the field since 1996, especially the application of mass spectrometry in protein characterization and the 2-D PAGE technique in proteomics. A section on glycoprotein is substantially expanded, and methods for the production of single chains and phage-displayed antibodies have been added. Each method offers a concise summary of its basic theory, a materials list, a step-by-step protocol, and notes on avoiding pitfalls and modifying the method to individual circumstances. Most authors are the originators of the techniques described. Walker is affiliated with the University of Hertfordshire, UK. Annotation c. Book News, Inc., Portland, OR (booknews.com)From The Critics
Reviewer: Eugene A Davidson, PhD(Georgetown University School of Medicine)Description: This is a compilation of methods used in protein analysis and characterization. The 164 contributions cover almost all aspects of protein identification. This second edition (first edition published in 1996) contains much new material, especially in areas relating to proteomics.
Purpose: The book is intended as a laboratory companion for investigators working with proteins. The intent is to provide protocols with sufficient detail so as to allow their ready adaptation in most working laboratories. These are useful objectives and generally well met.
Audience: It is targeted to graduate students, fellows, and senior investigators — in short, those in the laboratory environment. The multiauthor list includes very well known authorities with worldwide representation. The editor has done a fine job in assembling this group.
Features: A book that intends to provide complete protocol coverage for scientists working with proteins must, of necessity, be large. This contribution is no exception and might have profited from more critical editing. For example, it is not essential to detail six general methods for estimating protein concentration when no more than two of these are currently in routine use. Nevertheless, the breadth of techniques covered is large and this alone makes the book of practical value. There are 164 chapters in all, divided into seven major sections. In addition to quantitation, areas covered include electrophoretic methods, blotting, chemical modification, glycoproteins and antibody methods (with a separate section on monoclonal antibodies). Each of the chapters has a detailed protocol for the given procedure; bibliographic citations range from negligible to adequate. The protocols are provided with sufficient detail so as to allow implementation as new methods in any working laboratory. In most cases, a brief discussion of the theory behind the method provides useful material, especially for students. In addition, pitfalls and limitations are usually indicated, an important asset to any collection of protocols. It can easily be envisioned that this book would become the subject of a tug of war in a busy laboratory. Most investigators will find value here as will students working in almost any area dealing with proteins.
Assessment: This is a compilation not found in other single sources. The very broad scope has considerable practical value. I personally want this in my laboratory.
3 Stars from Doody