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Overview
Adenoviruses have become the premier and prolific model to study cellular biology because they are benign to the researcher, easy to grow and manipulate, stable, versatile, and very interesting. They are also important for transforming cells to an oncogenic state.This prominent researchers that have contributed to this volume provide true step-by-step adenovirus (Ad) protocols covering most aspects of current Ad research. The first seven chapters describe how to grow and titer Ads, and to construct specific alterations in the Ad genome. This is followed by five chapters detailing how to measure apoptosis induced by cells of the immune system, cytokines, and intrinsic apoptosis effectors. Next come in vitro systems to study transcription and splicing, followed by seven concluding chapters dealing with specialized topics of interest to beginning and experienced researchers.
The book contains black-and-white illustrations.
Editorials
From The Critics
Reviewer:Rebecca T. Horvat, PhD, D(ABMM)(University of Kansas Medical Center)Description:As the title implies, this book provides details on the procedures that can be used to construct adenoviruses to be used as vectors. Each chapter specifically details laboratory procedures that can be used for these purposes.
Purpose:The book is intended to provide a "detailed description" of the methods used to grow, detect, and analyze adenoviruses. The overall purpose of growing and detecting these viruses is so that they can be used as vectors to transfer genes into cells or larger animals. This is of great interest now, since this could potentially provide a means of delivering genetic material as a drug.
Audience:Scientists working in the area of virology and gene transfer are the intended audience, but the book would be easy reading for most levels from the undergraduate student to the professional scientist. All the authors clearly have personal experience in using these techniques.
Features:The 18 chapters cover primarily the procedures used to grow adenoviruses and genetically manipulate the virus as well as the use of animal models to test the manipulated viruses. Each chapter starts with a summary and an introductory section, followed by a section on materials needed to perform the experimental protocols and then by specific methods to accomplish the experiments using adenoviruses. These sections are followed by notes on the procedures and are linked to the procedure by a number. Overall there is a great deal of information on laboratory technique. Some authors list very similar techniques, which can beconfusing for readers trying to make decisions on protocols. It was difficult to continually go back to the notes section and then find where you left the procedure. It would help to place the notes close to where the reference is in the protocol. Also, some of the techniques seem dated and might be hard to perform in a laboratory just starting in this area. Overall, though, the book provides some good advice on using and growing adenovirus vectors.
Assessment:This book would be useful for scientists in this area. A change in the size and format of the book would help. It does not stay open very well and at a lab bench, this could be annoying. It would also be useful to have the hints and notes embedded into the methods, making it clearer for the user.
Booknews
Comprises 25 contributions on methods for conducting research with adenoviruses. Among the topics: preparation and titration of CsCl-banded adenovirus stock, methods for creating and analyzing adenovirus vectors that express proteins that act on the viral genome, measurement of macrophage-mediated cytolysis of adenovirus-infected cells, and phylogenetic analysis of adenovirus sequences. Annotation c. by Book News, Inc., Portland, Or.Book Details
Published
August 28, 1998
Publisher
Totowa, NJ : Humana Press, c1999.
Pages
352
Format
Hardcover
ISBN
9780896035515