Books.org participates in affiliate programs including Bookshop.org and the Amazon Services LLC Associates Program. We may earn a commission from qualifying purchases made through links on this page, at no additional cost to you.
Overview
Biochemical Techniques in the Heart fully describes classical biochemical measurements of parameters involved in contraction and relaxation in the heart. This fully detailed guide tells you how to make preparations of sarcoplasmic reticulum, sarcolemma, and cardiomyocytes and how to measure sodium-calcium and sodium-hydrogen exchange. Two chapters explain the measurement of the important enzyme sodium-potassium ATPase.
This book examines the most widely used tools in experimental cardiology and provides you with the recipe-setting up the technique, procurement of equipment, sample data and calculations, problems and trouble shooting, adapting to other species, modifications, and applicability. Undoubtedly, this text will be a great asset to cardiovascular physiologists, pharmacologists, experimental cardiologists, and students of physiology and pharmacology.
The book contains black-and-white illustrations.
Synopsis
Biochemical Techniques in the Heart fully describes classical biochemical measurements of parameters involved in contraction and relaxation in the heart. This fully detailed guide tells you how to make preparations of sarcoplasmic reticulum, sarcolemma, and cardiomyocytes and how to measure sodium-calcium and sodium-hydrogen exchange. Two chapters explain the measurement of the important enzyme sodium-potassium ATPase.
This book examines the most widely used tools in experimental cardiology and provides you with the recipe-setting up the technique, procurement of equipment, sample data and calculations, problems and trouble shooting, adapting to other species, modifications, and applicability. Undoubtedly, this text will be a great asset to cardiovascular physiologists, pharmacologists, experimental cardiologists, and students of physiology and pharmacology.
Richard L. Clancy
This book describes techniques for preparing myocardial sarcoplasmic reticulum, sarcolemma, and cardiac myocytes. The remaining chapters are devoted to illustrating how these preparations are used to measure transmembrane ion fluxes. The objective is to provide a ""recipe book"" for the investigator who has little or no experience in using these types of cardiac cell and subcellular preparations. The fact that methodology sections of most peer-reviewed articles are by necessity brief makes this a valuable book. The completeness of the descriptions will aid the investigator in developing and using any one of these techniques. The descriptions are readily comprehensible for the laboratory assistant, the student, and the senior investigator. All the authors have extensive experience in using these myocyte preparations. Flow charts illustrating techniques for isolating cell and subcellular systems and tables detailing composition of solutions and assays are used very effectively. Each chapter has a sizable number of current and relevant references. Of concern are several pages in which the print is out of focus. This book is very useful to the investigator who has not had any experience using any one of these cardiac cell preparations. The book provides a comprehensive yet succinct description of how to isolate myocardial cells and cellular components, the equipment, supplier and solutions needed for performing experiments using these preparations, and the advantages and disadvantages of each type of preparation. Biomedical libraries should be encouraged to purchase a copy of this book.
Editorials
From The Critics
Reviewer: Richard L. Clancy, PhD(University of Kansas Medical Center)Description: This book describes techniques for preparing myocardial sarcoplasmic reticulum, sarcolemma, and cardiac myocytes. The remaining chapters are devoted to illustrating how these preparations are used to measure transmembrane ion fluxes.
Purpose: The objective is to provide a "recipe book" for the investigator who has little or no experience in using these types of cardiac cell and subcellular preparations. The fact that methodology sections of most peer-reviewed articles are by necessity brief makes this a valuable book. The completeness of the descriptions will aid the investigator in developing and using any one of these techniques.
Audience: The descriptions are readily comprehensible for the laboratory assistant, the student, and the senior investigator. All the authors have extensive experience in using these myocyte preparations.
Features: Flow charts illustrating techniques for isolating cell and subcellular systems and tables detailing composition of solutions and assays are used very effectively. Each chapter has a sizable number of current and relevant references. Of concern are several pages in which the print is out of focus.
Assessment: This book is very useful to the investigator who has not had any experience using any one of these cardiac cell preparations. The book provides a comprehensive yet succinct description of how to isolate myocardial cells and cellular components, the equipment, supplier and solutions needed for performing experiments using these preparations, and the advantages and disadvantages of each type of preparation. Biomedical libraries should be encouraged to purchase a copy of this book.
Richard L. Clancy
This book describes techniques for preparing myocardial sarcoplasmic reticulum, sarcolemma, and cardiac myocytes. The remaining chapters are devoted to illustrating how these preparations are used to measure transmembrane ion fluxes. The objective is to provide a ""recipe book"" for the investigator who has little or no experience in using these types of cardiac cell and subcellular preparations. The fact that methodology sections of most peer-reviewed articles are by necessity brief makes this a valuable book. The completeness of the descriptions will aid the investigator in developing and using any one of these techniques. The descriptions are readily comprehensible for the laboratory assistant, the student, and the senior investigator. All the authors have extensive experience in using these myocyte preparations. Flow charts illustrating techniques for isolating cell and subcellular systems and tables detailing composition of solutions and assays are used very effectively. Each chapter has a sizable number of current and relevant references. Of concern are several pages in which the print is out of focus. This book is very useful to the investigator who has not had any experience using any one of these cardiac cell preparations. The book provides a comprehensive yet succinct description of how to isolate myocardial cells and cellular components, the equipment, supplier and solutions needed for performing experiments using these preparations, and the advantages and disadvantages of each type of preparation. Biomedical libraries should be encouraged to purchase a copy of this book.3 Stars from Doody