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Overview
The importance of capillary electrophoresis (CE) as an analytical tool has increased dramatically over the last ten years. It has changed from being an exploratory technique, mainly of academic interest, to one that is applied to solve "real" analytical problems. CE is easily adapted to its various modes of operation, often requiring little more than a change of the buffer solution, and is quickly becoming the preferred technique when analyzing minute amounts of available material.
Featuring new chapters on CE analysis of inorganic ions and carbohydrates, the new edition of Capillary Electrophoresis not only presents this method as an academic tool, but also provides applications for solving "real-world" analytical problems. This updated Second Edition reflects the increasing use of CE over the last 10 years, how it is being applied, and the basic theoretical aspects of the separation and detection methodology of CE. Capillary Electrophoresis: Theory and Practice will appeal to students and professionals of analytical chemistry, physical chemistry, biochemistry, and biotechnology and includes suitable experiments designed to be attempted by university or college students, or anyone else wishing to familiarize themselves with CE.
The book contains black-and-white illustrations.
Editorials
From The Critics
Reviewer: Juergen Mollenhauer, PhD, DSc(Rush Medical College of Rush University)Description: This is an extremely useful collection of theoretical background, practical applications, and important advice given by a 14 coauthors. These chapters cover everything from inorganic ions to large macromolecules and from plain separation to advanced characterization of molecules. The collection is completed by three appendixes on equipment and manufacturers, buffer solutions, and some model experiments, as well as a very detailed reference base.
Purpose: Its purpose is to provide detailed information on all aspects of capillary electrophoresis.
Audience: It is written for experts in molecular analysis, but also for teachers at colleges and universities who intend to introduce capillary electrophoresis.
Features: Very detailed illustrative support in the form of diagrams and electrophoresis profiles provides a great deal of clarity to the individual chapters and facilitates understanding even for scientists or teachers who are not yet familiar with the technique. Very frequently, the molecular structures of the molecules to be separated are presented: an excellent idea since the broad range of applications makes it otherwise very difficult to understand the basis for the particular separation.
Assessment: The book will be relevant at least for a decade. In other words, it is highly recommended for anyone who intends to use capillary electrophoresis in her/his laboratory or teachers who have to educate future laboratory rats how to crack scientific nuts.
Juergen Mollenhauer
This is an extremely useful collection of theoretical background, practical applications, and important advice given by a 14 coauthors. These chapters cover everything from inorganic ions to large macromolecules and from plain separation to advanced characterization of molecules. The collection is completed by three appendixes on equipment and manufacturers, buffer solutions, and some model experiments, as well as a very detailed reference base. Its purpose is to provide detailed information on all aspects of capillary electrophoresis. It is written for experts in molecular analysis, but also for teachers at colleges and universities who intend to introduce capillary electrophoresis. Very detailed illustrative support in the form of diagrams and electrophoresis profiles provides a great deal of clarity to the individual chapters and facilitates understanding even for scientists or teachers who are not yet familiar with the technique. Very frequently, the molecular structures of the molecules to be separated are presented: an excellent idea since the broad range of applications makes it otherwise very difficult to understand the basis for the particular separation. The book will be relevant at least for a decade. In other words, it is highly recommended for anyone who intends to use capillary electrophoresis in her/his laboratory or teachers who have to educate future laboratory rats how to crack scientific nuts.Booknews
Outlines the basic theoretical aspects of the separation and detection methodology and demonstrates applications of the technique for analyzing a range of analytes differing widely in properties such as size, charge, and hydrophobicity. Discusses the history and development of the field; separating small organic molecules; interfacing with mass spectrometry and other detection techniques; and separating proteins, peptides, and oligonucleotides. No date is noted for the first edition, but the second includes new chapters on inorganic ions and carbohydrates. Appended are a list of suppliers, tables of a number of suitable buffer solutions, and experiments suitable for college students and others just learning the technique. Annotation c. by Book News, Inc., Portland, Or.4 Stars! from Doody