Overview
This book provides a concise yet comprehensive account of animal cell cultures: how they are used in cell growth assays, how to interpret results, and how to avoid potential problem areas when using these commonly employed research tools. Cell Growth and Division furnishes laboratory protocols for using a wide range of animal cell lines. The book gives readers full coverage on measuring parameters of growth, mouse embryo cells, C3H 10T1/2 and NIHT3T cell lines, human leukemia cells, and normal human epidermal keratinocytes and mesothelial cells. This complete guide also offers convenient, all-in-one coverage of cloned T cell proliferation, muscle cell cultures, human diploid fibroblasts, cell synchronization, and BAL B/c3T3 cells. Laboratory investigators familiar with only one cell line will particularly appreciate the depth of coverage, which will make it possible to choose the line best suited to a given investigation. guidance on how they are carried out, how to interpret results, and how to avoid potential problem areas when using these commonly employed research tools.