Basic Sciences, Chemistry - General and miscellaneous, Biology & Life Sciences, Applied Science, Science - Applied, Biochemistry, Chemistry, Biology, Chemistry - Biochemistry
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Overview
Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today.This universal method is applicable to most microscopical systems including optical microscopy, scanning, transmission and high voltage electron microscopy, and photoelectron, photon, fluorescent darkfield and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection.
Key Features
* Among the first books to cover principles and methodology of colloidal gold in microscopy
* Describes methods step by step to enable researchers to learn these complex procedures solely by reference to the book
* Discusses problems and limitations of techniques
* Guides users to avoid problems and choose the correct procedures for specific applications
* Contributors are eminent authorities in their fields
Topics covered in Volume 1 include:
* Principles, methods, and applications of colloidal gold methodology in cytochemistry and immunochemistry
* Methods for preparing colloidal gold particles of different sizes
* Protein A-gold, protein G-gold, and lectin-gold techniques
* The use of resins and thin cryosections
* Multiple labeling
Topics covered in Volume 2 include:
* Methodology for the study of antigenicity in neurology, virology, exocytosis, cytoskeleton, and plant tissues.
* The useof colloidal gold in conjunction with negative staining, nucleic acid hybridization, and darkfield and epipolarization microscopy
* The detection of proteins with colloidal gold
Molecular interactions between gold and proteins
* The roles of tissue processing in colloidal gold methods
* Low temperature methods
Topics covered in Volume 3 include:
* Production of monoclonal and polyclonal antibodies
* Lowicryl, polar, and apolar resins
* Preserving and localizing antigenicity; sectioning; high-resolution morphological localization
* Diaminobenzidine-colloidal gold; microinjection of colloidal gold; double labeling with backscattered electrons; microwave irradiation
* Applications to biomedical studies
* Colloidal gold in conjunction with video-enhanced microscopy
Audience: Researchers, technicians, teachers, and students in cell biology, immunocytochemistry, histochemistry, and electron microscopy.
Editorials
Booknews
Colloidal gold, introduced in 1971, has become the method of choice for diverse applications in cell biology and immunocytochemistry. This universal method is applicable to most microscopical systems, including optical microscopy; scanning, transmission and high voltage electron microscopy; and photoelectron, photon, fluorescent darkfield and epipolarization microscopy. This third volume completes Hayat's coverage of principles and methodology. Annotation c. Book News, Inc., Portland, OR (booknews.com)Book Details
Published
December 28, 1990
Publisher
Elsevier Science & Technology Books
Pages
421
Format
Hardcover
ISBN
9780123339294