Differential Display Methods and Protocols
Peng Liang (Editor), Jonathan Meade (Editor), Arthur B. PardeeBooks.org participates in affiliate programs including Bookshop.org and the Amazon Services LLC Associates Program. We may earn a commission from qualifying purchases made through links on this page, at no additional cost to you.
Overview
Comprehensive review of this new technology, including methodology and practical applications. For investigators studying gene expression regulation. Plastic-comb, spiral binding.The book contains black-and-white illustrations.
Synopsis
Carrying on the high standards of the first edition of Differential Display Methods, Peng Liang et al. have based their second edition on a new mathematical model of differential display (DD) that takes advantage of automation, as well as digital data acquisition and analysis. These well-versed authors explain and highlight all the recent methodological refinements, including automated liquid handling of hundreds of DD PCR reaction setups combined with capillary electrophoresis, a prototype computer program to automatically allow positive band identification from a fluorescence differential display image, and restriction fragment-based DD screenings that can link any cDNA fragment directly to a given gene once the sequence information of all transcripts becomes available. Other improvements discussed are combining DD and DNA microarrays by reducing the complexity of cDNA probes while increasing the sensitivity of detection, and a DD approach to detect prokaryotic mRNA expression. The authors also demonstrate the power of DD technology with a collection of outstanding examples of DD applications and detailed experimental procedures. The elegant studies described here have led to the discovery of many important genes involved in viral infection, Prion disease, cancer, ovulation, circadian clock, floral color, transcription repression gene silencing, mRNA polymorphism, and protein-RNA interaction.
State-of-the-art and highly practical, Differential Display Methods, Second Edition offers gene hunters the possibility of genome-wide comprehensive DD screening, as well as a proven road map for any successful "gene fishing" expedition.
Philip M. Sass
This is a well thought-out and presented book that covers one of the most important molecular biology technologies developed, differential display. It is intended to provide an authoritative treatise on differential display so that researchers who are either unfamiliar with or are having problems can take advantage of the technology. The protocols that are included are well described and should be easy to follow for those with a basic grasp of molecular biology. The book is organized into several parts, including a basic methods section in the beginning of the book, which is followed by a series of parts that describe the application of differential display to various biological questions. These parts include sections on cloning family-specific genes and those genes expressed during normal development and during disease development. Furthermore, there are many helpful sections that follow each of the chapters that make the process of trouble-shooting fairly straightforward. I would highly recommend this book to libraries and to individuals who want to apply differential display technology to their research.
Editorials
From The Critics
Reviewer: Eugene A Davidson, PhD(Georgetown University School of Medicine)Description: This is the second edition (first edition published in 1997) of a compilation of protocols for investigators working with differential display. Both theoretical and practical aspects are addressed; several practical applications are included.
Purpose: The goal is to provide a set of protocols for investigators wishing to use differential display methods for monitoring gene expression. There are competing methods, notably array technologies, and the differences/advantages are not discussed in detail. The goal is worthwhile, but overall not well achieved.
Audience: This is essentially a practical laboratory manual and as such is intended for the senior graduate student/research fellow/investigator. There is sufficient detail to allow most of the experiments to be executed. The editors have assembled a diverse and capable group of contributors.
Features: Given the current knowledge of the genome and the ability to measure gene expression in multiple ways, it is to be expected that investigators will want to follow the "performance" of one or more genes during a variety of cellular events. These include differentiation/development, malignant transformation, sequelae of infectious processes, etc. Although the use of microarrays is very popular, these are high cost items and not available to all laboratories. Thus, alternative approaches are desirable. This book offers a series of protocols aimed at providing the tools necessary to use differential display. In principle, messenger RNA can be prepared from the tissue of interest, a cDNA copy made and then specific interrogation made of that population by PCR or other techniques. The initial chapter provides a theoretical discussion of global gene display addressing issues such as coverage of the chromosome when targets are not explicit. Subsequent chapters are laboratory oriented and include methods for automation, use of ordered displays, and PCR methods designed for this approach. The final section includes several explicit studies such as detection of genes expressed in prion disease, ovulation, and those related to p53. Each chapter has a short reference list to complement the detailed instructions. As a second edition, there is some new material. However, some sections are not very successful. Thus, the discussion of one new gene possibly identified in prion disease belongs in the primary literature awaiting confirmation. The automated procedure described is very challenging experimentally and, as with other chapters, insufficiently describes expected pitfalls and experimental snags. Investigators needing to use differential display will find useful, albeit limited, material.
Assessment: As a second edition, this book does not add very much. It is particularly important for a book of laboratory protocols to include caveats else the inexperienced investigator may be left at sea. At the same time, the experienced laboratorian is aware of the issues. The book seems cobbled together rather than tightly organized.
Philip M. Sass
This is a well thought-out and presented book that covers one of the most important molecular biology technologies developed, differential display. It is intended to provide an authoritative treatise on differential display so that researchers who are either unfamiliar with or are having problems can take advantage of the technology. The protocols that are included are well described and should be easy to follow for those with a basic grasp of molecular biology. The book is organized into several parts, including a basic methods section in the beginning of the book, which is followed by a series of parts that describe the application of differential display to various biological questions. These parts include sections on cloning family-specific genes and those genes expressed during normal development and during disease development. Furthermore, there are many helpful sections that follow each of the chapters that make the process of trouble-shooting fairly straightforward. I would highly recommend this book to libraries and to individuals who want to apply differential display technology to their research.Booknews
Twenty-four contributions describe all the major elements of this novel technology, including both RAP-PCR and DD using fluorescence detection, as well as a strategy for identifying and cloning family- specific genes. The protocols provide examples in which differentially expressed genes were successfully identified in diverse biological systems, as well as new tools for studying how gene expression is regulated throughout the development of a living organism, and how the failure of this control mechanism leads to pathological complications. Plastic comb binding. Annotation c. by Book News, Inc., Portland, Or.5 Stars! from Doody