Overview
This new 4-part companion volume set of Practical Approach titles covers the major techniques required by all modern molecular biology laboratories. Each volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories. For example, transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organization and expression, in vitro mutagenesis, and DNA sequencing are covered. The first two volumes are thoroughly revised versions of the original volumes published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories. Volume 3 covers a variety of cloning techniques for complex genome studies and covers the use of cosmid clones, cloning into YACS, databases, and long-range restriction mapping. The fourth and last v olume in the set, restriction mapping. The fourth and last volume in the set, describes a variety of techniques for genetic manipulation of mammalian cells. Topics covered include construction of vaccinia virus recominants, retroviral vectors, production of transgenic animals, expressions using the HSV-1 vector systems, and adenovirus vectors. The information provided in these four volumes comprises a completely up-to-date account of each topic by established researchers.