Forensic DNA Profiling Protocols (Methods in Molecular Biology Series, Volume 98)

Leading forensic experts from around the world describe in detail their time-proven methods for identity testing through DNA analysis. Their state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The book covers PCR- based test systems, the now widely used STR typing systems (complete with details of both manual and automated detection systems), repeat unit mapping (MVR), direct phase minisequencing, capillary electrophoresis, and direct blotting. Also fully discussed are the recovery of DNA from a wide range of sample types (blood, semen, skeletal remains, and saliva), species testing, sex determination, and mitochondrial DNA testing.

This state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The methods include the recovery of DNA from a large range of sample types, analysis of DNA as single and multi-locus VNTR probes, PCR amplification of STR and other loci, and mitochondrial sequencing. The expert scientists writing here-many from laboratories around the world-also discuss how to interpret the results in cases of unknown identity and disputed parentage.

Booknews

Describes the techniques of DNA identity-testing. The text is very specific, detailing exactly what chemicals and procedures are to be used for a variety of situations, from analysis of saliva-stained materials like cigarette butts and stamps to higher-quality samples like those taken directly from blood or other parts of the body. Most chapters deal with PCR typing, but other methods such as VNTR and Southern blot analysis are also covered. Annotation c. by Book News, Inc., Portland, Or.

Lincoln, Patrick J. (Univ of London); Thomson, Jim