G Protein-Coupled Receptor-Protein Interactions

This exciting volume elucidates how GPCRs organize signal transduction and control intracellular activities. Chapters written by the recognized expert in each respective topic offer state-of-the-art updates on the principles and technology, as well as stepwise protocols for methods currently applied to the analysis of protein-receptor interactions.

G protein-coupled receptors, the largest family of cell surface receptors, are responsible for mediating the signals from a wide group of signaling molecules. The study of their signaling complex and signaling pathways, which dynamically regulate so many aspects of intracellular function, has spawned a number of methodologies in biochemistry and cell biology for investigating interactions between receptors and between receptors and proteins, and the 16 papers here survey such methods. They explain the principles guiding the method, procedural details, and practical tips. Among the methods are time-resolved fluorescence resonance energy to analyze receptor-receptor interactions, and the yeast two-hybrid system to detect receptor-protein interactions. Annotation ©2004 Book News, Inc., Portland, OR

SUSAN R. GEORGE, PhD, MD, FRCP, FACP, is Professor in the Departments of Medicine and Pharmacology at the University of Toronto and a Section Head of Molecular Pharmacology at the Centre for Addiction and Mental Health, Toronto.

BRIAN F. O'DOWD, PhD, is Professor in the Department of Pharmacology at the University of Toronto and a Section Head of Molecular Pharmacology at the Centre for Addiction and Mental Health, Toronto.