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Overview
Here is a newly updated and expanded edition of a useful and popular guide. In addition to providing new information on topics covered in the first edition, this volume covers a number of major techniques which must be mastered by molecular biologists and biochemists. All the main aspects of polyacrylamide gel electrophoresis are described, while valuable appendix materials bring the reader useful listings of suppliers' addresses, detection methods, and more.
Synopsis
This new edition is almost a completely new text, with eight of the ten chapters written by new authors. It presents the most reliable methods for essential procedures such as one-dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements of the procedures. In addition, it describes major new techniques developed since the previous edition. These include capillary gel electrophoresis, sequence analysis of gel-resolved proteins, fluorophore-labelled saccharide electrophoresis, and analysis of protein-protein interactions by gel electrophoresis. Like the previous editions, this volume is easy-to-follow and thorough, a laboratory manual written by experienced researchers for researchers. The emphasis is on describing the best methods, in step-by-step detail, with copious advice to ensure that each method works the first time. This new edition is a 'must' for anyone currently uses gel electrophoresis.
Booknews
A laboratory manual presenting methods in a step-by-step format, and hints and tips from experienced researchers. Among the varieties of gel electrophoresis described are one-dimensional polyacrylamide; isoelectric, two-dimensional; and preparative. Eight of the ten chapters of the earlier editions, in 1981 and 1990, have been rewritten by new authors. They discuss methods that have been developed and tested since then, including capillary gel electrophoresis, the sequence analysis of gel-resolved proteins, fluorophore-labelled saccharide electrophoresis, and analyzing interactions between proteins. Annotation c. Book News, Inc., Portland, OR (booknews.com)