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Overview
With new technologies emerging and old technologies being reimagined, the field of hematopoietic stem cell (HSC) research continues to grow rapidly in pursuit of improved therapies for many blood diseases. Updating and building upon the thorough first edition's foundation, Hematopoietic Stem Cell Protocols, Second Edition provides up-to-date protocols developed in the HSC field. A team of leading researchers supply this volume with in-depth, readily reproducible methods for effective characterization of HSC and their developmental potential. An introductory chapter also hints at potential techniques that may develop in the future. Following the format of the successful Methods in Molecular Biology series, each protocol offers step-by-step laboratory instructions, lists of the necessary equipment, and tips on troubleshooting and avoiding known pitfalls. Essential for the laboratory-based researcher, Hematopoietic Stem Cell Protocols, Second Edition is a much needed technical resource in the critically important field of stem cell investigation.Synopsis
With new technologies emerging and old technologies being reimagined, the field of hematopoietic stem cell (HSC) research continues to grow rapidly in pursuit of improved therapies for many blood diseases. Updating and building upon the thorough first edition's foundation, Hematopoietic Stem Cell Protocols, Second Edition provides up-to-date protocols developed in the HSC field. A team of leading researchers supply this volume with in-depth, readily reproducible methods for effective characterization of HSC and their developmental potential. An introductory chapter also hints at potential techniques that may develop in the future. Following the format of the successful Methods in Molecular Biology™ series, each protocol offers step-by-step laboratory instructions, lists of the necessary equipment, and tips on troubleshooting and avoiding known pitfalls.
Essential for the laboratory-based researcher, Hematopoietic Stem Cell Protocols, Second Edition is a much needed technical resource in the critically important field of stem cell investigation.
Doody Review Services
Reviewer:Bruce A. Fenderson, PhD(Thomas Jefferson University)
Description:Hematopoiesis is a complex developmental process characterized by stem cell renewal, lineage formation, and differentiation. This book, part of the user-friendly Methods in Molecular Biology series, provides detailed protocols for the identification, isolation, genetic manipulation, and differentiation of hematopoietic stem cells. These progenitor cells can be obtained from cultured embryonic stem (ES) cells, fetal tissues, and adult bone marrow. The book includes 20 chapters on topics ranging from stem cell homing to hematopoietic development of human ES cells. The primary focus is on cellular and developmental biology in human and mouse model systems.
Purpose:According to the editor, the aim of this book is to provide an "essential technical resource" for laboratory-based researchers interested in clinical applications of hematopoietic stem cells. His stated goal is to provide "updated approaches for the combination of phenotypic and functional analyses.
Audience:The book is written for basic science and clinical researchers interested in stem cell biology, hematology, and transplantation medicine. Biomedical scientists and clinicians interested in immunology, oncology, and regenerative medicine will also appreciate the wealth of information and exciting new ideas presented in this concise, hardcover book.
Features:Five major sections present an overview and information on stem cell enrichment, in vitro assays, transplantation assays, and genetic modification of hematopoietic stem cells. Each chapter includes a summary, introduction, list of essential materials and equipment, and step-by-step instructions for proven laboratory techniques. The authors present examples of results from their laboratories, such as protocols for flow cytometric analysis of mixed stem cell populations. Numerous line drawings, figures, and tables highlight key laboratory findings. A few figures are presented in color. The popular "Notes" section at the end of each chapter provides practical tips for achieving success in the laboratory. Each chapter concludes with a list of key references. The authors are experts in this clinically relevant and rapidly advancing field.
Assessment:The authors assume that readers are familiar with the basic biology of hematopoiesis, as well as current methods for cell sorting. The book builds on basic methods published in the first edition. In this second edition, the editor and authors highlight examples of cutting-edge research and emphasize new technology. Advances in stem cell biology will provide clinicians with new approaches to treating patients with congenital and acquired hematopathologies. The protocols in this book provide useful tools that will stimulate basic research.
Editorials
From The Critics
Reviewer: Bruce A. Fenderson, PhD(Thomas Jefferson University)Description: Hematopoiesis is a complex developmental process characterized by stem cell renewal, lineage formation, and differentiation. This book, part of the user-friendly Methods in Molecular Biology series, provides detailed protocols for the identification, isolation, genetic manipulation, and differentiation of hematopoietic stem cells. These progenitor cells can be obtained from cultured embryonic stem (ES) cells, fetal tissues, and adult bone marrow. The book includes 20 chapters on topics ranging from stem cell homing to hematopoietic development of human ES cells. The primary focus is on cellular and developmental biology in human and mouse model systems.
Purpose: According to the editor, the aim of this book is to provide an "essential technical resource" for laboratory-based researchers interested in clinical applications of hematopoietic stem cells. His stated goal is to provide "updated approaches for the combination of phenotypic and functional analyses."
Audience: The book is written for basic science and clinical researchers interested in stem cell biology, hematology, and transplantation medicine. Biomedical scientists and clinicians interested in immunology, oncology, and regenerative medicine will also appreciate the wealth of information and exciting new ideas presented in this concise, hardcover book.
Features: Five major sections present an overview and information on stem cell enrichment, in vitro assays, transplantation assays, and genetic modification of hematopoietic stem cells. Each chapter includes a summary, introduction, list of essential materials and equipment, and step-by-step instructions for proven laboratory techniques. The authors present examples of results from their laboratories, such as protocols for flow cytometric analysis of mixed stem cell populations. Numerous line drawings, figures, and tables highlight key laboratory findings. A few figures are presented in color. The popular "Notes" section at the end of each chapter provides practical tips for achieving success in the laboratory. Each chapter concludes with a list of key references. The authors are experts in this clinically relevant and rapidly advancing field.
Assessment: The authors assume that readers are familiar with the basic biology of hematopoiesis, as well as current methods for cell sorting. The book builds on basic methods published in the first edition. In this second edition, the editor and authors highlight examples of cutting-edge research and emphasize new technology. Advances in stem cell biology will provide clinicians with new approaches to treating patients with congenital and acquired hematopathologies. The protocols in this book provide useful tools that will stimulate basic research.