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Overview
Interpreting Protein Mass Spectra is a hands-on laboratory guide for a wide range of researchers investigating the structures of proteins and peptides. The focus is on the interpretation of structural information gathered through electrospray ionization-mass spectrometry (ESI-MS). The book will also provide useful background and protocols for anyone using matrix-assisted laser desorption/ionization (MALDI), fast atom bombardment (FAB), and secondary ion mass spectrometry (SIMS). The book includes numerous practical examples, detailed discussions of experimental setups, and valuable hints for troubleshooting both methods and the handling of materials.
The book contains black-and-white illustrations.
Synopsis
Interpreting Protein Mass Spectra is a hands-on laboratory guide for a wide range of researchers investigating the structures of proteins and peptides. The focus is on the interpretation of structural information gathered through electrospray ionization-mass spectrometry (ESI-MS). The book will also provide useful background and protocols for anyone using matrix-assisted laser desorption/ionization (MALDI), fast atom bombardment (FAB), and secondary ion mass spectrometry (SIMS). The book includes numerous practical examples, detailed discussions of experimental setups, and valuable hints for troubleshooting both methods and the handling of materials.
Eugene A. Davidson
The introduction of electrospray ionization into mass spectrometry has permitted direct analysis of proteins. This sensitive and powerful technique will be a major contributor to proteomic studies. This serves as combination text, primer, and manual for those in the field. This book could be used as a text for graduate students or a primer for investigators wishing to familiarize themselves with the power of the methodology. The book begins with an introduction to the technology and continues with detailed examples of protein spectra. The latter sections are presented as a series of problems with ample illustrations designed to guide the reader through the interpretive process. The book concludes with chapters on phospho- and glycoproteins, major types of post-translational modifications. Two appendixes add technical information on experimental conditions, isotope abundances and common peptides and proteins. Each chapter is accompanied by a useful bibliography. Those wishing to understand and, perhaps, interpret such macromolecular data will be well rewarded by using this as a study guide. There are some minor errors in the glycoprotein section regarding the effect of alkali, but they do not seriously diminish the overall utility of the volume.
Editorials
Eugene A. Davidson
The introduction of electrospray ionization into mass spectrometry has permitted direct analysis of proteins. This sensitive and powerful technique will be a major contributor to proteomic studies. This serves as combination text, primer, and manual for those in the field. This book could be used as a text for graduate students or a primer for investigators wishing to familiarize themselves with the power of the methodology. The book begins with an introduction to the technology and continues with detailed examples of protein spectra. The latter sections are presented as a series of problems with ample illustrations designed to guide the reader through the interpretive process. The book concludes with chapters on phospho- and glycoproteins, major types of post-translational modifications. Two appendixes add technical information on experimental conditions, isotope abundances and common peptides and proteins. Each chapter is accompanied by a useful bibliography. Those wishing to understand and, perhaps, interpret such macromolecular data will be well rewarded by using this as a study guide. There are some minor errors in the glycoprotein section regarding the effect of alkali, but they do not seriously diminish the overall utility of the volume.From The Critics
Reviewer:Eugene A Davidson, PhD (Georgetown University School of Medicine)Description:The introduction of electrospray ionization into mass spectrometry has permitted direct analysis of proteins. This sensitive and powerful technique will be a major contributor to proteomic studies.
Purpose:This serves as combination text, primer, and manual for those in the field.
Audience:This book could be used as a text for graduate students or a primer for investigators wishing to familiarize themselves with the power of the methodology.
Features:The book begins with an introduction to the technology and continues with detailed examples of protein spectra. The latter sections are presented as a series of problems with ample illustrations designed to guide the reader through the interpretive process. The book concludes with chapters on phospho- and glycoproteins, major types of post-translational modifications. Two appendixes add technical information on experimental conditions, isotope abundances and common peptides and proteins. Each chapter is accompanied by a useful bibliography.
Assessment:Those wishing to understand and, perhaps, interpret such macromolecular data will be well rewarded by using this as a study guide. There are some minor errors in the glycoprotein section regarding the effect of alkali, but they do not seriously diminish the overall utility of the volume.