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Overview
Laser microdissection techniques have revolutionized the ability of researchers in general, and pathologists in particular, to carry out molecular analysis on specific types of normal and diseased cells and to fully utilize the power of current molecular technologies including PCR, microarrays, and proteomics. In second edition of Laser Capture Microdissection: Methods and Prools, experts in the field provide the reader with practical advice on how to carry out tissue-based laser microdissection successfully in their own laboratory using the different laser microdissection systems that are available and to apply a wide range of molecular technologies. The individual chapters encompass detailed descriptions of the individual laser based micro-dissection systems. The downstream applications of the laser microdissected tissue described in the book include PCR in its many different forms as well as gene expression analysis including application to microarrays and proteomics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Laser Capture Microdissection: Methods and Prools, Second Edition is an ideal resource for researchers striving to move forward our understanding of normal physiology and pathology.
Editorials
From The Critics
Reviewer: Mirna Lechpammer, MD, PhD(Rhode Island Hospital)Description: This book offers a comprehensive overview of current applications of laser microdissected tissue ranging from DNA and RNA analysis to applications in proteomics and molecular analysis of microorganisms.
Purpose: The book is conceptualized to provide readers with practical guidance on how to successfully use the available laser capture microdissection (LCM) systems in their own laboratories and how best to apply a wide range of molecular technologies. The editors have thus addressed a growing need for a comprehensive set of protocols, providing a valuable tool for wide range of researchers.
Audience: Due to its unique concept, covering methods applied from basic histology and cancer research to microbiology, this book is an equally useful guide for undergraduate students, postdoctoral fellows, and research scientists in academia and industry likewise.
Features: The editors have gathered an impressive international panel of contributors to cover a variety of applications of laser microdissected tissue including polymerase chain reaction (PCR) in its many different forms and gene expression analysis involving microarrays and proteomics. The individual chapters encompass detailed descriptions of several currently available LCM systems. However, the authors do not provide critical comparison of these systems, but rather provide practical guidance on each of them to meet individual needs of prospective readers. The chapters thus contain complete protocols on tissue preparation and DNA and/or RNA analysis, and are adequately illustrated with both the LCM histologic images and the results of biochemical analyses.
Assessment: Overall, this book will successfully meet the expectations of readers searching for a step-by-step laboratory cookbook and for the guidance in a growing use of the LCM technology in contemporary research.
4 Stars! from Doody