Membrane Protein Purification and Crystallization: A Practical Guide
Carola Hunte (Editor), Hermann Schagger (Editor), Gebhard von JagowBooks.org participates in affiliate programs including Bookshop.org and the Amazon Services LLC Associates Program. We may earn a commission from qualifying purchases made through links on this page, at no additional cost to you.
Overview
This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins.Key features
* Provides general guidelines and strategies for isolation and crystallization of membrane proteins
* Gives detailed protocols that have wide application, and low specialized equipment needs
* Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins
* Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes
* Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization
* Presents a comprehensive guide to 2D-crystallization of membrane proteins
Audience: Biochemists, molecular, cell, and structural biologists, and researchers in the pharmaceutical and biotechnical industry.
Synopsis
This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first manual compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins.
Key Features
* Provides guidelines for isolation and crystallization of membrane proteins
* Presents a comprehensive guide to 2D-crystallization of membrane proteins
* Describes production and use of antibody fragments
* Emphasizes recent progress in purification of recombinant membrane proteins
* Gives detailed protocols
New Contributions to the Second Edition
* An entire section devoted to crystallization of membrane proteins
* Summarizes recent developments of Blue-Native PAGE
* Production and purification of recombinant membrane proteins
* Preparative Isoelectric Focussing
* Purification of affinity-epitope tagged G-protein coupled receptors
* Purification of NhaA, the Na+/H+ Antiporter of Escherichia coli for 3D or 2D crystallization
* Purification of the yeast cytochrome bc1 complex
The Second Edition contains 75% new content!