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Biology - Microbiology, Infectious Diseases, Biology - Molecular Biology, Genetics - General and Miscellaneous, Biology - Bacteriology, Microbiology, Diagnosis
Molecular Bacteriology: Protocols and Clinical Applications by Woodford, Neil , Johnson, Alan β€” book cover

Molecular Bacteriology: Protocols and Clinical Applications

by Woodford, Neil, Johnson, Alan
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Overview

In Molecular Bacteriology: Prools and Clinical Applications, Woodford and Johnson bring together in a single volume a state-of-the-art collection of detailed molecular methods for the diagnosis and clinical investigation of bacterial infections. These wide ranging techniques-each presented by recognized experts who have fine-tuned their methods for optimum performance-will permit readers to establish them successfully in their own laboratories. An accompanying series of minireviews highlights and explains the clinical and epidemiological relevance of the techniques and shows how they may be applied to major pathogen species and various types of antibiotic resistance.

Molecular Bacteriology: Prools and Clinical Applications enlarges our current understanding of the role that molecular methodology can play in modern medical bacteriology, fully explaining both the practical and theoretical aspects of the cutting-edge methods that are increasingly finding diagnostic use in laboratories throughout the world. An authoritative laboratory resource for everyone working with infectious diseases today, this book's insightful reviews and detailed prools will soon win many devotees.

The book contains black-and-white illustrations.

Synopsis

The enormous advances in molecular biology that have been witnessed in . Not recent years have had major impacts on many areas of the biological sciences least of these has been in the field of clinical bacteriology and infectious disease . Molecular Bacteriology: Protocols and ClinicalApplications aims to provide the reader with an insight into the role that molecular methodology has to play in modern medical bacteriology. The introductory chapter ofMolecular Bacteriology: ProtocolsandCli- cal Applications offers a personal overview by a Consultant Medical Microbio- gist of the impact and future potential offered by molecular methods. The next six chapters comprise detailed protocols for a range of such methods . We believe that the use of these protocols should allow the reader to establish the various methods described in his or her own laboratory. In selecting the methods to be included in this section, we have concentrated on those that, arguably, have greatest current relevance to reference clinical bacteriology laboratories; we have deliberately chosen not to give detailed protocols for certain methods, such as multilocus enzyme electrophoresis that, in our opinion, remain the preserve of specialist la- ratories and that are not currently suited for general use. We feel that the methods included in this section will find increasing use in diagnostic laboratories and that it is important that the concepts, advantages, and limitations of each are th- oughly understood by a wide range of workers in the field .

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Editorials

Eugene M. Muller

This is a collection of articles that discuss the molecular biological methods that now, or soon will, affect the fields of clinical microbiology and infectious diseases and bring about significant changes. The publication of this manual is timely; it is a readable review of the most important techniques and their application for the study of the major bacterial pathogens of humans. In the cases in which detailed protocols are not presented, sufficient literature citations are provided. Although intended primarily for clinical microbiologists and infectious disease specialists, this book will be an excellent reference for investigators who want to use established molecular techniques in a variety of studies. The numerous contributing authors are well regarded within their respective fields and their descriptions and interpretations of their techniques will allow readers to efficiently establish state-of-the-art methodologies in their laboratories. The introductory chapter discusses the impact of molecular methods on bacteriology; six chapters specifically discuss the current methodologies in use (ribotyping, pulsed-field gel electrophoresis, plasmid analysis, DNA amplification and polymerase chain reaction, genomic fingerprinting) ; seventeen chapters address the major groups of bacterial diseases of humans; and seven chapters address antibiotic resistance. Each chapter contained in this volume is current and well written, and supported with an extensive and recent list of citations. This is an important reference for molecular techniques that will soon change clinical microbiology. It will be a valuable addition to the collection of libraries that serve medical and academiccommunities. It is an essential addition to the private libraries of clinical microbiologists and those studying infectious diseases.

Booknews

This book provides a wide range of techniques for the diagnosis and investigation of bacterial infections. In addition to the diagnostic and treatment procedures, some of the 31 contributions also show how molecular methods can be applied to different bacterial pathogens, diseases, and antimicrobial resistances. The Notes sections included at the ends of most of the chapters should prove to be of particular interest, as they often include tricks of the trade that the various contributors have learned through personal experience. Annotation c. by Book News, Inc., Portland, Or.

From The Critics

Reviewer: Eugene M. Muller, PhD(Framingham State College)
Description: This is a collection of articles that discuss the molecular biological methods that now, or soon will, affect the fields of clinical microbiology and infectious diseases and bring about significant changes.
Purpose: The publication of this manual is timely; it is a readable review of the most important techniques and their application for the study of the major bacterial pathogens of humans. In the cases in which detailed protocols are not presented, sufficient literature citations are provided.
Audience: Although intended primarily for clinical microbiologists and infectious disease specialists, this book will be an excellent reference for investigators who want to use established molecular techniques in a variety of studies. The numerous contributing authors are well regarded within their respective fields and their descriptions and interpretations of their techniques will allow readers to efficiently establish state-of-the-art methodologies in their laboratories.
Features: The introductory chapter discusses the impact of molecular methods on bacteriology; six chapters specifically discuss the current methodologies in use (ribotyping, pulsed-field gel electrophoresis, plasmid analysis, DNA amplification and polymerase chain reaction, genomic fingerprinting) ; seventeen chapters address the major groups of bacterial diseases of humans; and seven chapters address antibiotic resistance. Each chapter contained in this volume is current and well written, and supported with an extensive and recent list of citations.
Assessment: This is an important reference for molecular techniques that will soon change clinical microbiology. It will be a valuable addition to the collection of libraries that serve medical and academic communities. It is an essential addition to the private libraries of clinical microbiologists and those studying infectious diseases.

4 Stars! from Doody

Book Details

Published
November 9, 2010
Publisher
Springer-Verlag New York, LLC
Pages
694
Format
Paperback
ISBN
9781617370533

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