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Overview
Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Alan Gerstein and his collaborators have been guiding researchers of all levels of expertise through the troubleshooting process over the course of three decades, assuring that Molecular Biology Problem Solver will prove an excellent resource for all life scientists.Synopsis
Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as:
* Have you selected the most appropriate research strategy?
* Have you identified the issues critical to your successful application of a technique?
* Are you familiar with the limitations of a given technique?
* When should common procedural rules of thumb not be applied?
* What strategies could you apply to resolve a problem?
A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include:
* Working safely with biological samples and radioactive materials
* DNA and RNA purification
* PCR
* Protein and nucleid acid hybridization
* Prokaryotic and eukaryotic expression systems
* Properly using and maintaining laboratory equipment
Booknews
This guide features 16 contributions from technical support group professionals addressing issues commonly brought up by life science researchers when requesting assistance. A sampling of topics includes properly using and maintaining laboratory equipment, dealing with suppliers, working safely with biological samples and radioactive materials, and understanding DNA and RNA purification, and Western blotting techniques. Gerstein is with Amersham Pharmacia Biotech in Piscataway, NJ. Annotation c. Book News, Inc., Portland, OR
Editorials
This guide features 16 contributions from technical support group professionals addressing issues commonly brought up by life science researchers when requesting assistance. A sampling of topics includes properly using and maintaining laboratory equipment, dealing with suppliers, working safely with biological samples and radioactive materials, and understanding DNA and RNA purification, and Western blotting techniques. Gerstein is with Amersham Pharmacia Biotech in Piscataway, NJ. Annotation c. Book News, Inc., Portland, OR