Myeloid Leukemia: Methods and Protocols

A panel of internationally recognized research scientists and clinical investigators brings together a diverse collection of readily reproducible methods for identifying and quantifying a large number of specific genetic abnormalities associated with the broad spectrum of myeloid malignancies. Highlights include techniques for the detection of BCR-ABL mutations and resistance to imatinib mesylate, detection of the FIP1L1-PDGFRA fusion in idiopathic hypereosinophilic syndrome and chronic eosinophilic leukemia, classification of AML by DNA-oligonucleotide microarrays, and detection of the V617F JAK2 mutation in myeloproliferative disorders. In addition to gene rearrangments, other prognostically relevant molecular lesions such as FLT3 mutations and WT-1 overexpression are covered.

Forty-eight international research scientists and clinical investigators contribute 18 chapters reviewing the laboratory techniques of benefit to laboratory scientists and hematologists in the investigation and management of patients with myeloid malignancies. Coverage includes techniques for the detection of BCR-ABL mutations and resistance to imatinib mesylate, detection of the FIP 1L1-PDGFRA fusion in idiopathic hypereosinophilic syndrome and chronic eosinophilic leukemia, classification of acute myeloid leukemia by DNA- oligonucleotide microarrays, detection of the V617F JAK2 mutation in myeloproliferative disorders, gene rearrangements, FLT3 mutations andWT-1 overexpression. Each protocol includes step-by- step laboratory instructions, an introduction explaining principles behind the techniques, list of necessary equipment and reagents, and tips for troubleshooting and avoiding known pitfalls. Illustrated with b&w diagrams, charts and tables. Annotation © 2006 Book News, Inc., Portland, OR