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Overview
Although there has been an explosion of interest and technology in the study of neural stem cells, many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. Neural Stem Cells: Methods and Protocols, Second Edition, revises and expands on the successful first edition in order to provide the most current, cutting-edge methods for scientists working to answer these questions. Using these step-by-step, readily reproducible laboratory protocols will allow investigators to produce pure populations that can serve as a means for understanding the biology of neural stem cells and adapting them for transplantation into disease models. In addition to the topics covered in the first edition, this new edition provides recently developed alternative methods for both human and rodent NSCs, along with chapters on karyotyping, migration, apoptosis, and NSC markers. Following the Methods in Molecular Biology series format, each chapter has an extended introduction for background, a list of necessary materials, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and current, Neural Stem Cells: Methods and Protocols, Second Edition, is an excellent source of information and inspiration for scientists longing to use these powerful cells against neurogenetic and neurodegenerative diseases.
Synopsis
Although there has been an explosion of interest and technology in the study of neural stem cells, many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. Neural Stem Cells, 2nd Edition revises and expands upon the successful first edition in order to provide the most current, cutting-edge methods of today for the scientists working to answer these questions. The use of these step-by-step, readily reproducible laboratory protocols will allow investigators to produce pure populations that can serve as a means of understanding the biology of neural stem cells and adapting them for transplantation into disease models. In addition to the topics covered in the first edition, this new volume provides recently developed alternative methods for both human and rodent NSCs, along with chapters on karyotyping, migration, apoptosis, and NSC markers. Following the Methods in Molecular Biology™ series format, each chapter has an extended introduction for background, a list of necessary materials, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and current, Neural Stem Cells, 2nd Edition is an excellent source of information and inspiration for scientists longing to use these powerful cells against neurogenetic and neurodegenerative diseases.
Doody Review Services
Reviewer:Celso Agner, MD, MSc(University of Illinois at Chicago College of Medicine)
Description:Neural stem cells have been the focus of much research in an attempt to better understand the nature and behavior of these cells in vivo. In addition, potential cure of degenerative neurological disorders has challenged both scientists and the lay community. Since the first edition of this book, much has changed in the laboratory techniques and understanding of neural stem cells. A second edition was, therefore, much needed.
Purpose:The purpose is to clarify research protocols in the investigation of neural stem cells. These worthy objectives are met.
Audience:Neurologists, neurosurgeons, and neuroscientists are the main audience.
Features:Divided into four sections, the book addresses cell culture, cellular, molecular, and transplantation techniques. Despite being primarily a technical book outlining many bench laboratory techniques, the last section describes a possible use for stem cells in the management of neurological conditions in the future. Neural stem cell transplantation may find its way to control neoplasms, aggressive multiple sclerosis, Parkinson's disease, and other conditions in the decades to come.
Assessment:This is a valuable acquisition for academic neuroscience, neurology, and neurosurgery libraries.
Editorials
From The Critics
Reviewer: Celso Agner, MD, MS, MSc(Michigan Neurology Partners)Description: Neural stem cells have been the focus of much research in an attempt to better understand the nature and behavior of these cells in vivo. In addition, potential cure of degenerative neurological disorders has challenged both scientists and the lay community. Since the first edition of this book, much has changed in the laboratory techniques and understanding of neural stem cells. A second edition was, therefore, much needed.
Purpose: The purpose is to clarify research protocols in the investigation of neural stem cells. These worthy objectives are met.
Audience: Neurologists, neurosurgeons, and neuroscientists are the main audience.
Features: Divided into four sections, the book addresses cell culture, cellular, molecular, and transplantation techniques. Despite being primarily a technical book outlining many bench laboratory techniques, the last section describes a possible use for stem cells in the management of neurological conditions in the future. Neural stem cell transplantation may find its way to control neoplasms, aggressive multiple sclerosis, Parkinson's disease, and other conditions in the decades to come.
Assessment: This is a valuable acquisition for academic neuroscience, neurology, and neurosurgery libraries.