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Overview
An outstanding collection of detailed, state-of-the-art techniques for studying many aspects of nervous system cell biology. The methods span a multidisciplinary range of cellular and molecular approaches to both normal and injured nervous system function, and particularly to neurodegenerative processes. Included are basic RT-PCR techniques, cell culture systems, second-messenger signaling methods, and patch-clamp techniques. These powerful techniques will illuminate nervous system function, injury, degeneration, and the repair/regenerative process.
The book contains black-and-white illustrations.
This book presents step-by-step protocols for the study of neurodegenerative diseases in both in-vivo and in-vitro model systems. Chapters cover laboratory techniques for the study of neural cell apoptosis and necrosis as well as the molecular mechanisms for apoptosis and programmed cell death. This research is important for understanding the mechanisms behind the major neurodegenerative diseases, including Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis (ALS).