Neurotransmitter Methods

N. Rao Gundamraj

This volume in the Methods in Molecular Biology series gives an informative account of the various laboratory techniques and protocols involving the localization or measurement of neurotransmitters. The methods are described in a lucid, easily understood recipe format. The methods of localizing various neurotransmitters in different locations of the brain are elaborated. The aim of this book is to assist in the identification of neurotransmitters and their measurement using the latest techniques and protocols. The format is user friendly and clear. The authors attempt to explain the various procedures step-by-step, starting with the basic techniques. This book is intended for neuroscientists working with neurotransmitters. It can also be a good reference for neurophysiologists, pharmacologists, psychiatrists, molecular biologists, neurologists, and neurosurgeons involved in research studies on brain tissues. Each chapter has a short introduction describing the basic information about the techniques and transmitters, followed by a list of the materials and the actual laboratory methods of evaluation.. The authors have used the latest techniques of molecular biology and genetic evaluations. This book is an excellent step-by-step approach to localization of neurotransmitters in brain tissue. The language is simple and the presentation is excellent. The book begins with basic techniques of slice preparation in the brain. Inclusion of the topic of synaptosomes is an added advantage to the readers. Variable approaches in cell and organ culture, in situ hybridization, cell fractionation etc. are used. This book is an excellent reference for anyone working with neurotransmitters. Thereferences are reliable and up-to-date. The diagrams and pictures are of good quality and are labeled appropriately. The techniques used are the latest in this field of study. This book not only serves as a comprehensive text of experimental methods for the practicing neuroscientist but is also a good aid for beginners in this field.

From The Critics

Reviewer: N. Rao Gundamraj, MD(University of Illinois at Chicago College of Medicine)
Description: This volume in the Methods in Molecular Biology series gives an informative account of the various laboratory techniques and protocols involving the localization or measurement of neurotransmitters. The methods are described in a lucid, easily understood recipe format. The methods of localizing various neurotransmitters in different locations of the brain are elaborated.
Purpose: The aim of this book is to assist in the identification of neurotransmitters and their measurement using the latest techniques and protocols. The format is user friendly and clear. The authors attempt to explain the various procedures step-by-step, starting with the basic techniques.
Audience: This book is intended for neuroscientists working with neurotransmitters. It can also be a good reference for neurophysiologists, pharmacologists, psychiatrists, molecular biologists, neurologists, and neurosurgeons involved in research studies on brain tissues.
Features: Each chapter has a short introduction describing the basic information about the techniques and transmitters, followed by a list of the materials and the actual laboratory methods of evaluation.. The authors have used the latest techniques of molecular biology and genetic evaluations. This book is an excellent step-by-step approach to localization of neurotransmitters in brain tissue. The language is simple and the presentation is excellent. The book begins with basic techniques of slice preparation in the brain. Inclusion of the topic of synaptosomes is an added advantage to the readers. Variable approaches in cell and organ culture, in situ hybridization, cell fractionation etc. are used.
Assessment: This book is an excellent reference for anyone working with neurotransmitters. The references are reliable and up-to-date. The diagrams and pictures are of good quality and are labeled appropriately. The techniques used are the latest in this field of study. This book not only serves as a comprehensive text of experimental methods for the practicing neuroscientist but is also a good aid for beginners in this field.

3 Stars from Doody