Overview
The first and only guide to showcase the impact of phage display technology on drug discovery, this reference details the theories, principles, and methods impacting the field and demonstrates applications for peptide phage display, protein phage display, and the development of novel antibodies. Highlighting the current and future role of phage display in the development of protein therapeutics, this book provides a comprehensive overview that will prove invaluable to anyone researching recombinant antibodies.
Daniel E. Levy, editor of the Drug Discovery Series, is the founder of DEL BioPharma, a consulting service for drug discovery programs. He also maintains a blog that explores organic chemistry.
Synopsis
The first and only guide to showcase the impact of phage display technology on drug discovery, this reference details the theories, principles, and methods impacting the field and demonstrates applications for peptide phage display, protein phage display, and the development of novel antibodies. Highlighting the current and future role of phage display in the development of protein therapeutics, this book provides a comprehensive overview that will prove invaluable to anyone researching recombinant antibodies.
Doody Review Services
Reviewer:Eugene A Davidson, PhD(Georgetown University School of Medicine)
Description:This book provides a set of reviews on various aspects of phage display technology. Coverage progresses from general introductory and background material to current applications.
Purpose:The goal is to provide an overview of the present status of phage display technology (principles as well as applications). Given the high interest in combinatorial methods, this is a worthy goal that, in general, is achieved.
Audience:Phage display is one of several combinatorial approaches presently used. The target audience includes scientists working in the area and those wishing to familiarize themselves with it. The editor has assembled a diverse and competent set of contributors.
Features:Phage display, first described about 20 years ago, was the first of the biotechnology-based combinatorial techniques available (a problem addressed eons earlier in the development of antibody responses). The diversity of expressed products derived from random DNA sequences is obviously huge and the corresponding implementation of high throughput screening methods allows for their exploitation for pharmaceutical development. This book provides a series of reviews on phage display techniques with an emphasis on their utility in drug development. The 17 chapters begin with a general discussion of the filamentous bacteriophage and useful vectors, library construction, and screening methods. Several chapters are devoted to specific applications such as antibody engineering, protein-protein interactions, and enzymatic optimization. Each section is accompanied by a comprehensive bibliography allowing easy access to primary sources. Although several similar treatises have appeared recently, the focus here is on the applications of phage display to therapeutic issues. This book will be of value to those working in this area as well as to investigators wishing an introduction to a powerful and increasingly used technology.
Assessment:This is a generally well-written set of articles on a well-known technique. Those not directly in the field can broaden their understanding of the breadth of applicability of this method. Although several similar books have recently appeared (O'Brien and Aitken, Antibody Phage Display: Methods and Protocols (Humana Press, 2002); Clackson and Lowman, Phage Display: A Practical Approach (Oxford University Press, 2004)), the organized coverage of this book is useful for almost any reader.
Editorials
From The Critics
Reviewer: Eugene A Davidson, PhD(Georgetown University School of Medicine)Description: This book provides a set of reviews on various aspects of phage display technology. Coverage progresses from general introductory and background material to current applications.
Purpose: The goal is to provide an overview of the present status of phage display technology (principles as well as applications). Given the high interest in combinatorial methods, this is a worthy goal that, in general, is achieved.
Audience: "Phage display is one of several combinatorial approaches presently used. The target audience includes scientists working in the area and those wishing to familiarize themselves with it. The editor has assembled a diverse and competent set of contributors. "
Features: Phage display, first described about 20 years ago, was the first of the biotechnology-based combinatorial techniques available (a problem addressed eons earlier in the development of antibody responses). The diversity of expressed products derived from random DNA sequences is obviously huge and the corresponding implementation of high throughput screening methods allows for their exploitation for pharmaceutical development. This book provides a series of reviews on phage display techniques with an emphasis on their utility in drug development. The 17 chapters begin with a general discussion of the filamentous bacteriophage and useful vectors, library construction, and screening methods. Several chapters are devoted to specific applications such as antibody engineering, protein-protein interactions, and enzymatic optimization. Each section is accompanied by a comprehensive bibliography allowing easy access to primary sources. Although several similar treatises have appeared recently, the focus here is on the applications of phage display to therapeutic issues. This book will be of value to those working in this area as well as to investigators wishing an introduction to a powerful and increasingly used technology.
Assessment: This is a generally well-written set of articles on a well-known technique. Those not directly in the field can broaden their understanding of the breadth of applicability of this method. Although several similar books have recently appeared (O'Brien and Aitken, Antibody Phage Display: Methods and Protocols (Humana Press, 2002); Clackson and Lowman, Phage Display: A Practical Approach (Oxford University Press, 2004)), the organized coverage of this book is useful for almost any reader.