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Overview
This wide-ranging collection of state-of-the-art techniques for the assay of phospholipid and phospholipid-derived second messengers allows the identification and quantification of signal pathway activation. The assays described cover all the major phospholipases (C, D, A2), as well as sphingomyelinase and associated metabolites. Additional prools are provided for the assay of phosphoinositide 3-, 4-, and 5-kinase activity and for the separation and identification of phospholipids, diacylglycerol and sphingolipids, as well as their phosphoinositol, choline, and fatty acid metabolites. Detailed, step-by-step instructions make this book suitable for both the newcomer and the experienced investigator.
The book contains black-and-white illustrations.
Synopsis
This wide-ranging collection of state-of-the-art techniques for the assay of phospholipid and phospholipid-derived second messengers allows the identification and quantification of signal pathway activation. The assays described cover all the major phospholipases (C, D, A2), as well as sphingomyelinase and associated metabolites. Additional protocols are provided for the assay of phosphoinositide 3-, 4-, and 5-kinase activity and for the separation and identification of phospholipids, diacylglycerol and sphingolipids, as well as their phosphoinositol, choline, and fatty acid metabolites. Detailed, step-by-step instructions make this book suitable for both the newcomer and the experienced investigator.
Booknews
Contributors detail techniques for monitoring the major lipid and phospholipid-derived second messenger pathways to identify and quantify pathway activation. The assays described cover all the major phospholipases (C, D, A2), as well as sphingomyelinase and its associated metabolites. Additional protocols are provided for the assay of phosphoinositide 3-, 4-, and 5-kinase and sphingosine kinase activity, and for the quantification, separation, and rigorous identification of phospholipids, diacylglycerol, and sphingolipids, as well as their metabolites. There is also extensive information on the extraction, size separation, detection, and quantification of cellular signaling proteins and corresponding mRNA. Annotation c. by Book News, Inc., Portland, Or.