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Overview
There have been many changes in techniques of plant analysis since the second edition of this guide was published in 1984. Most significant has been the increasingly widespread use of analytical HPLC as a routine technique in plant analysis and the increased sophistication of NMR and related spectral techniques. The third edition provides an outline and summary of up-to-date techniques for analyzing plants for their organic constituents. Particular procedures used routinely in many phytochemical laboratories are recommended and sufficient details are provided for students to use the techniques for themselves.Synopsis
This long awaited third edition of Phytochemical Methods is, as its predecessors, a key tool for undergraduates, research workers in plant biochemistry, plant taxonomists and any researchers in related areas where the analysis of organic plant components is key to their investigations.
Phytochemistry is a rapidly expanding area with new techniques being developed and existing ones perfected and made easier to incorporate as standard methods in the laboratory. This latest edition includes descriptions of the most up-to-date methods such as HPLC and the increasingly sophisticated NMR and related spectral techniques. Other methods described are the use of NMR to locate substances within the plant cell and the chiral separation of essential oils.
After an introductory chapter on methods of plant analysis, individual chapters describe methods of identifying the different type of plant molecules: phenolic compounds, terpenoids, organic acids, lipids and related compounds, nitrogen compounds, sugar and derivatives and macromolecules. Different methods are discussed and recommended, and guidance provided for the analysis of compounds of special physiological relevance such as endogenous growth regulators, substances of pharmacological interest and screening methods for the detection of substances for taxonomic purposes. It also includes an important bibliographic guide to specialized texts.
This comprehensive book constitutes a unique and indispensable practical guide for any phytochemistry or related laboratory, and provides hands-on description of experimental techniques so that students and researchers can become familiar with these invaluable methods.