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Overview
A comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus.
"...state-of-the-art protocols for cloning coat protein genes, an up-to-date review of plant resistance & current opinions about its mechanisms, & methods for generating & identifying cDNA clones representing the coat protein gene."
Synopsis
A comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus.
Booknews
A theoretical and hands-on guide to generating transgenic plants that are resistant to viruses via the cloning and expression of the coat protein gene. One hundred contributors cover plant virus purification protocols, protocols for cloning coat protein genes, methods for plant transformation and primary analysis, plant resistance and its mechanisms, techniques for analyzing viral genes and nucleotide sequences, methods for generating and identifying cDNA clones representing the coat protein gene, and RNA extraction. Annotation c. by Book News, Inc., Portland, Or.