Books.org participates in affiliate programs including Bookshop.org and the Amazon Services LLC Associates Program. We may earn a commission from qualifying purchases made through links on this page, at no additional cost to you.
Overview
Prools for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative prools required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive prool implemented in the laboratory with minimum adaptation required and fastest time to results.
The prools follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Synopsis
Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results.
The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Doody Review Services
Reviewer:Eugene A Davidson, PhD(Georgetown University School of Medicine)
Description:This is a set of protocols for various types of nucleic acid analysis using nonradioactive methods. The first edition was published in 1994.
Purpose:The goal is to provide protocols for nucleic acid analysis using other than radioactive reporter systems. This approach is commonplace at present, so a volume of practical utility has merit. There are gaps in coverage as well as trivial redundancies that reduce its effectiveness.
Audience:The target audience would be research laboratory personnel working in this general area. The editors have recruited a group of contributors with worldwide representation.
Features:There are numerous reasons for an investigator to want to characterize a particular nucleic acid sequence. In many cases, sensitivity is an important issue and thus appropriate reporter molecules are sought. Radioactive tracers have the requisite characteristics but equally efficient alternative means have been developed. This book provides protocols for commonly used methods wherein alternative means for readout are employed. The book is divided into several sections. Introductory material offers simple methods to prepare nucleic acid samples for subsequent analysis. Subsequent sections include discussions and protocols for several amplification techniques (PCR, enzymatic or immuno amplification), and the use of arrays. Each protocol has sufficient detail to allow for laboratory use; a short bibliography is also provided for each of the 19 chapters. Comparable how-to books are likewise available and the potential reader should examine alternatives in order to select the one best suited for the given laboratory. Some amplification methods are not described (use of phi29 polymerase, for example) and several of the protocols would appear to have limited utility.
Assessment:After an interval of 13 years, a more comprehensive effort might be expected. There are better sources for this material, especially for PCR and other DNA-amplification approaches.
Editorials
From The Critics
Reviewer: Eugene A Davidson, PhD(Georgetown University School of Medicine)Description: This is a set of protocols for various types of nucleic acid analysis using nonradioactive methods. The first edition was published in 1994.
Purpose: The goal is to provide protocols for nucleic acid analysis using other than radioactive reporter systems. This approach is commonplace at present, so a volume of practical utility has merit. There are gaps in coverage as well as trivial redundancies that reduce its effectiveness.
Audience: The target audience would be research laboratory personnel working in this general area. The editors have recruited a group of contributors with worldwide representation.
Features: There are numerous reasons for an investigator to want to characterize a particular nucleic acid sequence. In many cases, sensitivity is an important issue and thus appropriate reporter molecules are sought. Radioactive tracers have the requisite characteristics but equally efficient alternative means have been developed. This book provides protocols for commonly used methods wherein alternative means for readout are employed. The book is divided into several sections. Introductory material offers simple methods to prepare nucleic acid samples for subsequent analysis. Subsequent sections include discussions and protocols for several amplification techniques (PCR, enzymatic or immuno amplification), and the use of arrays. Each protocol has sufficient detail to allow for laboratory use; a short bibliography is also provided for each of the 19 chapters. Comparable how-to books are likewise available and the potential reader should examine alternatives in order to select the one best suited for the given laboratory. Some amplification methods are not described (use of phi29 polymerase, for example) and several of the protocols would appear to have limited utility.
Assessment: After an interval of 13 years, a more comprehensive effort might be expected. There are better sources for this material, especially for PCR and other DNA-amplification approaches.