Single Cell Diagnostics: Methods and Protocols

From The Critics

Reviewer: Bruce A. Fenderson, PhD(Thomas Jefferson University)
Description: This book details innovative methods for analyzing gene expression, chromosome organization, and proteins in single cells and cell extracts. The 13 chapters cover topics ranging from laser capture microdissection to fluorescence in situ hybridization on single cells to use of real time PCR to measure gene expression in single cells. The experimental protocols described in this book were developed initially to examine the blastomeres of human embryos for single gene mutations and chromosomal abnormalities. The primary focus of this book is on biochemistry and molecular biology.
Purpose: According to the editor, the aim "is for all readers to extend their knowledge and expertise in analysis of single cells." The authors detail methods for salvaging diagnostic information from single cells without contamination. The authors believe that this book will provide an excellent source of tips for diagnostic medicine, reproductive medicine, and experimental biology.
Audience: The book is written for basic science and clinical researchers, particularly those providing care to couples seeking advice regarding infertility and inherited genetic disorders. The editor and authors believe that the technology outlined in this book will become incorporated into routine diagnostic procedures for a wide spectrum of human conditions and diseases, including neoplasia.
Features: Each chapter provides a summary, introduction, list of materials, and detailed outline of experimental protocols. Notes at the end of each chapter provide tips that are an invaluable source of practical information. The authors provide examples of interesting results from their laboratories. The chapters are written by experts and the book is carefully edited. Line drawings and continuous tone figures primarily serve to illustrate conceptual points.
Assessment: This concise book is written for experts with a sophisticated knowledge of cell and molecular biology. The laboratory protocols and tips will be valued by laboratory scientists interested in forensic medicine, reproductive medicine, and developmental genetics. Future applications of this technology may revolutionize experimental biology, allowing researchers to analyze patterns of protein and gene expression in individual cells in developing embryos and rare adult tissues.