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Overview
It is now understood that the response of mammalian cells to a wide variety of potentially toxic agents may be intimately linked with many human diseases, including rheumatoid arthritis, ischemia, fever, infection, and cancer. In Stress Response: Methods and Prools, Stephen Keyse has assembled a diverse collection of readily reproducible methods devoted to the study of these varied and powerful responses. Written by leading researchers expert in the techniques they describe, these detailed methods cover the detection and assay of stress-induced damage, the activation of a wide range of signal transduction pathways by cellular stress, stress-induced gene expression, and stress protein function. To ensure experimental success, step-by-step guidance is provided for each method, along with details of reagents, equipment, and other requirements. The methods include both well-established techniques and new technologies at the leading edge of research. Wide ranging and highly practical, Stress Response: Methods and Prools provides a gold-standard bench manual for today's basic and clinical scientists working to understand how cells and tissues respond during physiological stress and in human disease.
The book contains black-and-white illustrations.
This book provides a series of methods and protocols dealing with various aspects of the mammalian cellular stress response. These include the assay and detection of stress induced damage, the activation of signal transduction by cellular stress, the regulation of gene transcription by stress and the physiological function of stress proteins in mammalian cells. This book brings together in one volume a series of concise and proven methods which can be used to study the complex responses in mammalian cells, and will be of interest to both basic scientists working in cell and molecular biology and clinical researchers interested in the way in which cells and tissues respond during physiological stress and human disease.
Editorials
Michael B. Lilly
The realization that the mammalian stress response may be intimately linked with many human diseases, including rheumatoid arthritis, ischemia, fever, infection, and cancer, has led to an explosion of interest in this research area. This recent offering in the Methods in Molecular Biology series brings together a diverse array of practical methodologies that may be employed to address various aspects of the response of mammalian cells to environmental stress. The purpose is to present protocols that carry sufficient background information and experimental detail to be used as a primary reference at the laboratory bench. In addition the volume serves to highlight current areas of interest in stress response-related studies. As such the book succeeds reasonably well. A broad range of laboratory techniques, including methods for cell analysis, protein biochemistry, and molecular studies, are included. The primary audience is the laboratory worker who is becoming acquainted with the field of stress-related studies. Likely such researchers will find this volume quite useful. The volume editor, and most of the chapter authors, are well-known experts in the field, with immense personal experience in the procedures described. This book presents protocols grouped in four sections: detection of stress-induced damage, stress-induced signal transduction, stress-induced differential gene expression, and analysis of stress protein function. The abundant technical information in most chapters should allow adaptation of the protocols by anyone generally familiar with molecular biology. This volume emphasizes changes in protein activity or expression in response to various stresses. Curiously, there islittle information on methods for assessing changes in small molecule antioxidant systems (such as glutathione), redox systems, or lipid modifications. In addition the methods for analysis of differentially expressed genes are slightly dated by the inclusion of a chapter on differential display but none on suitable DNA microarrays. The chapters on SABRE and SAGE technologies for enrichment of differentially expressed cDNAs are timely and helpful, however. This should be a useful addition to laboratories moving into the areas of stress or oxidant response studies, particularly if they focus on kinases or protein modification.Booknews
Researchers describe new and established techniques they use to investigate possible links between the response of mammalian cells to a wide variety of potentially toxic agents, and a number of human diseases, among them rheumatoid arthritis, ischemia, fever, infection, and cancer. The techniques are concerned with detecting and assaying stress-induced damage, activating a wide range of signal transduction pathways by cellular stress, stress-induced gene expression, and stress protein function. Along with the steps-by-step procedures, they include details of reagents, equipment, and other requirements. Annotation c. Book News, Inc., Portland, OR (booknews.com)From The Critics
Reviewer: Michael B. Lilly, MD(Loma Linda University)Description: The realization that the mammalian stress response may be intimately linked with many human diseases, including rheumatoid arthritis, ischemia, fever, infection, and cancer, has led to an explosion of interest in this research area. This recent offering in the Methods in Molecular Biology series brings together a diverse array of practical methodologies that may be employed to address various aspects of the response of mammalian cells to environmental stress.
Purpose: The purpose is to present protocols that carry sufficient background information and experimental detail to be used as a primary reference at the laboratory bench. In addition the volume serves to highlight current areas of interest in stress response-related studies. As such the book succeeds reasonably well. A broad range of laboratory techniques, including methods for cell analysis, protein biochemistry, and molecular studies, are included.
Audience: The primary audience is the laboratory worker who is becoming acquainted with the field of stress-related studies. Likely such researchers will find this volume quite useful. The volume editor, and most of the chapter authors, are well-known experts in the field, with immense personal experience in the procedures described.
Features: This book presents protocols grouped in four sections: detection of stress-induced damage, stress-induced signal transduction, stress-induced differential gene expression, and analysis of stress protein function. The abundant technical information in most chapters should allow adaptation of the protocols by anyone generally familiar with molecular biology. This volume emphasizes changes in protein activity or expression in response to various stresses. Curiously, there is little information on methods for assessing changes in small molecule antioxidant systems (such as glutathione), redox systems, or lipid modifications. In addition the methods for analysis of differentially expressed genes are slightly dated by the inclusion of a chapter on differential display but none on suitable DNA microarrays. The chapters on SABRE and SAGE technologies for enrichment of differentially expressed cDNAs are timely and helpful, however.
Assessment: This should be a useful addition to laboratories moving into the areas of stress or oxidant response studies, particularly if they focus on kinases or protein modification.
3 Stars from Doody