Technologies for Detection of DNA Damage and Mutations

''Useful and timely.'' —-Mutagenesis ''Of considerable value.'' —-Journal of Medical Genetics ''Quite readable....a comprehensive overview....perfectly covers the needs of those researchers who have to decide on the best strategy to identify damage or mutations at the molecular level.'' —-Trends in Cell Biology ''The formats of the presentations are uniform and ample and up-to-date references are provided at the end of each chapter...will be welcomed by postgraduate researchers of all ages and should retain its usefulness for a long time.'' —-Endeavour, 21(4), 1997 This important resource thoroughly reviews a wide range of techniques used in mutagenesis research-ranging from established techniques to recently developed methodologies-based on the polymerase chain reaction. DNA damage analysis, DNA repair assays, and mutation detection are a few of the techniques featured. Chapters present detailed experimental prools benefiting researchers and students in the fields of toxicology, biotechniques, molecular biology, photobiology, medical genetics, and oncology.

Postlabeling assay for oxidative damage/methods to measure the repair of genes/heteroduplex analysis/sequencing of PCR.

''Useful and timely.'' —-Mutagenesis ''Of considerable value.'' —-Journal of Medical Genetics ''Quite readable....a comprehensive overview....perfectly covers the needs of those researchers who have to decide on the best strategy to identify damage or mutations at the molecular level.'' —-Trends in Cell Biology ''The formats of the presentations are uniform and ample and up-to-date references are provided at the end of each chapter...will be welcomed by postgraduate researchers of all ages and should retain its usefulness for a long time.'' —-Endeavour, 21(4), 1997 This important resource thoroughly reviews a wide range of techniques used in mutagenesis research-ranging from established techniques to recently developed methodologies-based on the polymerase chain reaction. DNA damage analysis, DNA repair assays, and mutation detection are a few of the techniques featured. Chapters present detailed experimental protocols benefiting researchers and students in the fields of toxicology, biotechniques, molecular biology, photobiology, medical genetics, and oncology.

Booknews

A collection of 31 discussions developing techniques useful in mutagenesis research, highlighting new technologies advanced through the advent of the polymerase chain reaction. The majority of the contributors supply experimental protocols in addition to explanations of rationale in the areas of methods for analyzing DNA damage and repair, damage frequencies in single cells or cell populations, damage distribution along gene sequences, methods of screening and sequence analysis, and complete mammalian mutagenesis systems using selectable genes in cell culture and living animals. Annotation c. Book News, Inc., Portland, OR (booknews.com)