Overview
The integration of confocal microscopy and volume investigation has led to an unprecedented ability to examine spatial relationships between cellular structure and function. The goal of this book is to familiarize the reader with these new technologies and to demonstrate their applicability to a wide range of biological and clinical problems.Key Features
* Volume investigation
* Three-dimensional reconstruction
* Fluroescent probe design
* Biological applications of confocal microscopy, including calcium imaging, receptor movement, and diagnostic pathology
* Confocal data display and analysis
* Twenty-eight pages of color
The book contains predominantly black-and-white illustrations, with some color illustrations.
Synopsis
The integration of confocal microscopy and volume investigation has led to an unprecedented ability to examine spatial relationships between cellular structure and function. The goal of this book is to familiarize the reader with these new technologies and to demonstrate their applicability to a wide range of biological and clinical problems.
Highlights
• Volume investigation
• Three-dimensional reconstruction
• Fluroescent probe design
• Biological applications of confocal microscopy, including calcium imaging, receptor movement, and diagnostic pathology
• Confocal data display and analysis
• Twenty-eight pages of color
Alvin Telser
This is a technically and theoretically oriented book on one of the newest and most important developments in optical microscopy in many years: laser scanning confocal light microscopy and its applications. It is a new book edited by a group of experts at the University of Toronto and a number of experts in the field. "The main purpose is to provide a thorough theoretical and practical basis for the use of the confocal microscope. It will be quite useful for the many investigators who use these techniques. The authors have made an excellent effort to cover theoretical matters such as the physics of photo multiplier tubes, charge coupled devices, and practical concerns such as stains and staining techniques. "The primary audience is researchers who use these techniques. It will also be a very useful reference book for anyone interested in the field. "The overall technical quality of the book is excellent. There are many graphs and line drawings of high quality. There are also several color sections that contain high quality images. "This is a valuable book for practitioners and libraries. The solid theoretical and practical sections will be very useful to the research community. The book also contains a few chapters describing other related approaches and techniques to 3-dimensional reconstruction techniques.
Editorials
From The Critics
Reviewer: Alvin Telser, PhD(Northwestern University Feinberg School of Medicine)Description: This is a technically and theoretically oriented book on one of the newest and most important developments in optical microscopy in many years: laser scanning confocal light microscopy and its applications. It is a new book edited by a group of experts at the University of Toronto and a number of experts in the field.
Purpose: The main purpose is to provide a thorough theoretical and practical basis for the use of the confocal microscope. It will be quite useful for the many investigators who use these techniques. The authors have made an excellent effort to cover theoretical matters such as the physics of photo multiplier tubes, charge coupled devices, and practical concerns such as stains and staining techniques.
Audience: The primary audience is researchers who use these techniques. It will also be a very useful reference book for anyone interested in the field.
Features: The overall technical quality of the book is excellent. There are many graphs and line drawings of high quality. There are also several color sections that contain high quality images.
Assessment: This is a valuable book for practitioners and libraries. The solid theoretical and practical sections will be very useful to the research community. The book also contains a few chapters describing other related approaches and techniques to 3-dimensional reconstruction techniques.
From The Critics
Reviewer: Alvin Telser, PhD(Northwestern University Feinberg School of Medicine)Description: This is a technically and theoretically oriented book on one of the newest and most important developments in optical microscopy in many years: laser scanning confocal light microscopy and its applications. It is a new book edited by a group of experts at the University of Toronto and a number of experts in the field.
Purpose: The main purpose is to provide a thorough theoretical and practical basis for the use of the confocal microscope. It will be quite useful for the many investigators who use these techniques. The authors have made an excellent effort to cover theoretical matters such as the physics of photo multiplier tubes, charge coupled devices, and practical concerns such as stains and staining techniques.
Audience: The primary audience is researchers who use these techniques. It will also be a very useful reference book for anyone interested in the field.
Features: The overall technical quality of the book is excellent. There are many graphs and line drawings of high quality. There are also several color sections that contain high quality images.
Assessment: This is a valuable book for practitioners and libraries. The solid theoretical and practical sections will be very useful to the research community. The book also contains a few chapters describing other related approaches and techniques to 3-dimensional reconstruction techniques.
Alvin Telser
This is a technically and theoretically oriented book on one of the newest and most important developments in optical microscopy in many years: laser scanning confocal light microscopy and its applications. It is a new book edited by a group of experts at the University of Toronto and a number of experts in the field. "The main purpose is to provide a thorough theoretical and practical basis for the use of the confocal microscope. It will be quite useful for the many investigators who use these techniques. The authors have made an excellent effort to cover theoretical matters such as the physics of photo multiplier tubes, charge coupled devices, and practical concerns such as stains and staining techniques. "The primary audience is researchers who use these techniques. It will also be a very useful reference book for anyone interested in the field. "The overall technical quality of the book is excellent. There are many graphs and line drawings of high quality. There are also several color sections that contain high quality images. "This is a valuable book for practitioners and libraries. The solid theoretical and practical sections will be very useful to the research community. The book also contains a few chapters describing other related approaches and techniques to 3-dimensional reconstruction techniques.Booknews
The capacity of confocal microscopy for obtaining high-resolution images of very thin sections allows researchers to look inside the cell at subcellular structures, and even dynamic events, previously invisible using conventional light microscopy. This book familiarizes readers with its potential for quantitative analysis of the 3- dimensional architecture of tissues, cells, organelles, and macro- molecular structures, a field newly defined as volume investigation. The applicability of these new technologies to a wide range of biological and clinical problems is also demonstrated. Annotation c. Book News, Inc., Portland, OR (booknews.com)4 Stars! from Doody