Overview
This work contains a concise exposition of the different immunocytochemical methods for identifying tissue constituents, discussing ways of preparing tissues according to the antigen to be localized and the most commonly used methods. The text is illustrated with explanatory diagrams and photomicrographs. An appendix contains step-by-step instructions for many of the basic techniques at light and electron microscopical levels. Other sections deal with sources of error and nonspecificity, and techniques for recording results photographically. For this revised edition, the authors have updated the text in light of the latest developments and added 20 new references to the literature.
The book contains black-and-white illustrations.
Editorials
Robert E. Lee
This second edition contains 11 chapters that consider requirements for immunocytochemistry specificity problems and controls, in vitro methods and applications of immunocytochemistry, and other topics. Each chapter is followed by references. A technical appendix and two pages of color plates are included. The book was first published in 1984. The authors state their hope is to provide a "practically directed basis for carrying out current immunocytochemical methods with enough theoretical information to allow a newcomer to understand the whys and wherefores of the technique." The objectives are worthwhile and are met in this book. The authors indicate this book is particularly written for those beginning to study or work in immunocytochemistry. This is accomplished. The authors are both staff members of the Hammersmith Hospitals Trust and the Royal Postgraduate Medical School, University of London. The tables and figures are suitable for this small book. The color plates are very limited but appropriate. The table of contents is detailed and the index is useful. The references cited are largely from the 1980s and 1990s (through 1996), with the 1960s and 1970s represented. This is a small book, neatly presented. Its convenient size lends itself to ease of consultation or reading. The appendix provides useful technical notes. The two authors have substantial expertise in this field based on years of teaching experience. This edition is substantially rewritten and updated. It remains a small but useful book, certainly for the novice, and a useful reference for the histotechnologist, pathologist, pathology resident, graduate student, and basic scientist.From The Critics
Reviewer: Robert E. Lee, MD, PhD (Loyola University Medical Center)Description: This second edition contains 11 chapters that consider requirements for immunocytochemistry specificity problems and controls, in vitro methods and applications of immunocytochemistry, and other topics. Each chapter is followed by references. A technical appendix and two pages of color plates are included. The book was first published in 1984.
Purpose: The authors state their hope is to provide a "practically directed basis for carrying out current immunocytochemical methods with enough theoretical information to allow a newcomer to understand the whys and wherefores of the technique." The objectives are worthwhile and are met in this book.
Audience: The authors indicate this book is particularly written for those beginning to study or work in immunocytochemistry. This is accomplished. The authors are both staff members of the Hammersmith Hospitals Trust and the Royal Postgraduate Medical School, University of London.
Features: The tables and figures are suitable for this small book. The color plates are very limited but appropriate. The table of contents is detailed and the index is useful. The references cited are largely from the 1980s and 1990s (through 1996), with the 1960s and 1970s represented. This is a small book, neatly presented. Its convenient size lends itself to ease of consultation or reading. The appendix provides useful technical notes.
Assessment: The two authors have substantial expertise in this field based on years of teaching experience. This edition is substantially rewritten and updated. It remains a small but useful book, certainly for the novice, and a useful reference for the histotechnologist, pathologist, pathology resident, graduate student, and basic scientist.
5 Stars! from Doody