Flow Cytometry Principles for Clinical Laboratory Practice: Quality Assurance for Quantitative Immunophenotyping
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Overview
FLOW CYTOMETRY PRINCIPLES FOR CLINICAL LABORATORY PRACTICE
Quality Assurance for Quantitative Immunophenotyping
Marilyn A. Owens and Michael R. Loken
Flow Cytometry Principles for Clinical Laboratory Practice is an invaluable benchtop reference and introduction for first-time users of flow cytometry in clinical diagnostic laboratories. This manual provides authoritative coverage of accepted methods, quality controls, and standards for the cytometric analysis of clinical samples with particular relevance to CD4+ lymphocytes. CD4+ lymphocyte determination by immunophenotyping is the standard method for evaluating the status of HIV infection, and the single most frequent application of flow cytometry in a diagnostic setting.
Flow Cytometry Principles for Clinical Laboratory Practice will appeal to clinical laboratory technologists, medical practitioners, and cytometry lab directors in their role as instructors of new personnel. In addition, it is a valuable resource for clinical and biomedical researchers, establishing quantitative cytometric systems for studies in immunology and hematology.
The book contains black-and-white illustrations.
Synopsis
FLOW CYTOMETRY PRINCIPLES FOR CLINICAL LABORATORY PRACTICE
Quality Assurance for Quantitative Immunophenotyping
Marilyn A. Owens and Michael R. Loken
Flow Cytometry Principles for Clinical Laboratory Practice is an invaluable benchtop reference and introduction for first-time users of flow cytometry in clinical diagnostic laboratories. This manual provides authoritative coverage of accepted methods, quality controls, and standards for the cytometric analysis of clinical samples with particular relevance to CD4+ lymphocytes. CD4+ lymphocyte determination by immunophenotyping is the standard method for evaluating the status of HIV infection, and the single most frequent application of flow cytometry in a diagnostic setting.
Flow Cytometry Principles for Clinical Laboratory Practice will appeal to clinical laboratory technologists, medical practitioners, and cytometry lab directors in their role as instructors of new personnel. In addition, it is a valuable resource for clinical and biomedical researchers, establishing quantitative cytometric systems for studies in immunology and hematology.
Joseph M. Pyle III
This is a new book written by two flow cytometry experts as a guide to quality assurance necessary for quantitative immunophenotyping by flow cytometry using clinical cases of CD4+ T cell enumeration and CD34+ stem cell quantitation in HIV infection and peripheral blood stem cell transplantation. The purpose is to describe the theory and practice of quality assurance in quantitative flow cytometric immunophenotyping in a ready-reference format for diagnostic laboratories for daily use. With accurate instrument maintenance and setup an absolute requirement for clinical sample analysis, this book does well to inform the laboratory personnel of the necessary procedures to ensure accurate results and above all, to comply with regulations applied to clinical laboratories using flow cytometers. Primarily intended for laboratory directors and laboratory technologists whose duties may include quantitative immunophenotyping by flow cytometry, this book may be recommended as a quick reference for other personnel desiring to troubleshoot an instrument that is performing suboptimally. The authors are experts in the field of flow cytometric analysis of peripheral blood who write very clearly at the intended level of their audience. The book is well referenced and each chapter concludes with a series of review questions that are useful in reviewing current laboratory procedures as well as self-assessment for laboratory personnel. The illustrations are bold histograms with individual legends as well as textual references. This is a laboratory guide to quality assurance in quantitative immunophenotyping of peripheral blood cells by flow cytometric analysis. It is concise, with detailed approaches to twofundamental clinical problems of quantitative CD4+ T cell counts in HIV disease and CD34+ peripheral stem cell counts in bone marrow transplantation. It is suggested for laboratory technologists, laboratory directors in larger hospitals, and medical school libraries.
Editorials
From The Critics
Reviewer: Joseph M. Pyle III, MD, PhD(Loyola University Medical Center)Description: This is a new book written by two flow cytometry experts as a guide to quality assurance necessary for quantitative immunophenotyping by flow cytometry using clinical cases of CD4+ T cell enumeration and CD34+ stem cell quantitation in HIV infection and peripheral blood stem cell transplantation.
Purpose: The purpose is to describe the theory and practice of quality assurance in quantitative flow cytometric immunophenotyping in a ready-reference format for diagnostic laboratories for daily use. With accurate instrument maintenance and setup an absolute requirement for clinical sample analysis, this book does well to inform the laboratory personnel of the necessary procedures to ensure accurate results and above all, to comply with regulations applied to clinical laboratories using flow cytometers.
Audience: Primarily intended for laboratory directors and laboratory technologists whose duties may include quantitative immunophenotyping by flow cytometry, this book may be recommended as a quick reference for other personnel desiring to troubleshoot an instrument that is performing suboptimally. The authors are experts in the field of flow cytometric analysis of peripheral blood who write very clearly at the intended level of their audience.
Features: The book is well referenced and each chapter concludes with a series of review questions that are useful in reviewing current laboratory procedures as well as self-assessment for laboratory personnel. The illustrations are bold histograms with individual legends as well as textual references.
Assessment: This is a laboratory guide to quality assurance in quantitative immunophenotyping of peripheral blood cells by flow cytometric analysis. It is concise, with detailed approaches to two fundamental clinical problems of quantitative CD4+ T cell counts in HIV disease and CD34+ peripheral stem cell counts in bone marrow transplantation. It is suggested for laboratory technologists, laboratory directors in larger hospitals, and medical school libraries.
Joseph M. Pyle III
This is a new book written by two flow cytometry experts as a guide to quality assurance necessary for quantitative immunophenotyping by flow cytometry using clinical cases of CD4+ T cell enumeration and CD34+ stem cell quantitation in HIV infection and peripheral blood stem cell transplantation. The purpose is to describe the theory and practice of quality assurance in quantitative flow cytometric immunophenotyping in a ready-reference format for diagnostic laboratories for daily use. With accurate instrument maintenance and setup an absolute requirement for clinical sample analysis, this book does well to inform the laboratory personnel of the necessary procedures to ensure accurate results and above all, to comply with regulations applied to clinical laboratories using flow cytometers. Primarily intended for laboratory directors and laboratory technologists whose duties may include quantitative immunophenotyping by flow cytometry, this book may be recommended as a quick reference for other personnel desiring to troubleshoot an instrument that is performing suboptimally. The authors are experts in the field of flow cytometric analysis of peripheral blood who write very clearly at the intended level of their audience. The book is well referenced and each chapter concludes with a series of review questions that are useful in reviewing current laboratory procedures as well as self-assessment for laboratory personnel. The illustrations are bold histograms with individual legends as well as textual references. This is a laboratory guide to quality assurance in quantitative immunophenotyping of peripheral blood cells by flow cytometric analysis. It is concise, with detailed approaches to twofundamental clinical problems of quantitative CD4+ T cell counts in HIV disease and CD34+ peripheral stem cell counts in bone marrow transplantation. It is suggested for laboratory technologists, laboratory directors in larger hospitals, and medical school libraries.4 Stars! from Doody