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Overview
This new edition of Animal Cell Culture covers new or updated chapters on cell authentication, serum-free culture, apoptosis assays, FISH, genetic modification, scale-up, stem cell assays, 3-dimensional culture, tissue engineering and cytotoxicity assays. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. Everyone working in biological and medical research, whether in academia or a commercial organization, practicing cell culture will benefit greatly from this book.
The book contains black-and-white illustrations.
Synopsis
This new edition of Animal Cell Culture covers new or updated chapters on cell authentication, serum-free culture, apoptosis assays, FISH, genetic modification, scale-up, stem cell assays, 3-dimensional culture, tissue engineering and cytotoxicity assays. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. Everyone working in biological and medical research, whether in academia or a commercial organization, practicing cell culture will benefit greatly from this book.
Valerie L. Ng
This is a combination reference book and lab manual for cell culture. It contains concise discussions of background, basic principles, and theory, accompanied by ""user-friendly"" lab protocols ready for direct application in the lab. This is a basic reference and practical lab manual resource for any laboratory using cell culture. This third edition is an update of previous editions and includes techniques and lab assays, developed within the past 5-10 years, now commonly in use in cell culture labs. This book contains useful information for anyone using cell culture. Beginning to mid-level graduate students learning to work with cell culture and bench level researchers who frequently rely on cell culture would especially benefit. This is a fairly comprehensive compendium of cell culture topics. The introductory chapter on various types of cell culture contains a nice comparison-contrast of monolayer, suspension, and immortalized versus primary cultures, and has a nice discussion of parameters affecting successful culture. New to this edition (and very welcome!) are chapters on stem cell culture, apoptosis, genetic manipulation and tissue engineering -- many of which contain ""lab-ready"" protocols. A really nice feature is the ""Table of Contents"" listing of all protocols in this text, allowing for quick access. I also liked the chapter on cell line authentication that contains appropriate warning and caution for researchers to verify the authenticity of their cell cultures, given the well-recognized problems with cell-culture cross-contamination and subsequent effects on research findings. This is a great book to have handy for daily use in any laboratory performing cellculture. It would also be very useful to those researchers considering cell culture based assays as a source of protocols ready for customization.
Editorials
From The Critics
Reviewer: Valerie L. Ng, PhD MD(Alameda County Medical Center/Highland Hospital)Description: This is a combination reference book and lab manual for cell culture. It contains concise discussions of background, basic principles, and theory, accompanied by "user-friendly" lab protocols ready for direct application in the lab.
Purpose: This is a basic reference and practical lab manual resource for any laboratory using cell culture. This third edition is an update of previous editions and includes techniques and lab assays, developed within the past 5-10 years, now commonly in use in cell culture labs.
Audience: This book contains useful information for anyone using cell culture. Beginning to mid-level graduate students learning to work with cell culture and bench level researchers who frequently rely on cell culture would especially benefit.
Features: This is a fairly comprehensive compendium of cell culture topics. The introductory chapter on various types of cell culture contains a nice comparison-contrast of monolayer, suspension, and immortalized versus primary cultures, and has a nice discussion of parameters affecting successful culture. New to this edition (and very welcome!) are chapters on stem cell culture, apoptosis, genetic manipulation and tissue engineering — many of which contain "lab-ready" protocols. A really nice feature is the "Table of Contents" listing of all protocols in this text, allowing for quick access. I also liked the chapter on cell line authentication that contains appropriate warning and caution for researchers to verify the authenticity of their cell cultures, given the well-recognized problems with cell-culture cross-contamination and subsequent effects on research findings.
Assessment: This is a great book to have handy for daily use in any laboratory performing cell culture. It would also be very useful to those researchers considering cell culture based assays as a source of protocols ready for customization.
Valerie L. Ng
This is a combination reference book and lab manual for cell culture. It contains concise discussions of background, basic principles, and theory, accompanied by ""user-friendly"" lab protocols ready for direct application in the lab. This is a basic reference and practical lab manual resource for any laboratory using cell culture. This third edition is an update of previous editions and includes techniques and lab assays, developed within the past 5-10 years, now commonly in use in cell culture labs. This book contains useful information for anyone using cell culture. Beginning to mid-level graduate students learning to work with cell culture and bench level researchers who frequently rely on cell culture would especially benefit. This is a fairly comprehensive compendium of cell culture topics. The introductory chapter on various types of cell culture contains a nice comparison-contrast of monolayer, suspension, and immortalized versus primary cultures, and has a nice discussion of parameters affecting successful culture. New to this edition (and very welcome!) are chapters on stem cell culture, apoptosis, genetic manipulation and tissue engineering -- many of which contain ""lab-ready"" protocols. A really nice feature is the ""Table of Contents"" listing of all protocols in this text, allowing for quick access. I also liked the chapter on cell line authentication that contains appropriate warning and caution for researchers to verify the authenticity of their cell cultures, given the well-recognized problems with cell-culture cross-contamination and subsequent effects on research findings. This is a great book to have handy for daily use in any laboratory performing cellculture. It would also be very useful to those researchers considering cell culture based assays as a source of protocols ready for customization.3 Stars from Doody