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Microscopes & Microscopy - Electron Microscopes, Optics - General & Miscellaneous
Biological Low-Voltage Scanning Electron Microscopy by James Pawley β€” book cover

Biological Low-Voltage Scanning Electron Microscopy

by James Pawley (Editor), Heide Schatten
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Overview

Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapters in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images.

Synopsis

Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. In FESEM, a field-emission cathode placed in the electron gun of a scanning electron microscope provides narrower probing beams and high electron energy. The result is improved spatial resolution and minimized sample charging and damage. Images produced are less destroyed and have a spatial resolution down to 1.5 nm, three to six times better than conventional SEM.

Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapter s in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images.

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Book Details

Published
January 1, 2008
Publisher
Springer-Verlag New York, LLC
Pages
328
Format
Hardcover
ISBN
9780387729701

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