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Overview
Over the course of the past decade, there have been remarkable advances in the study of human pathogenic fungi. These developments have taken place throughout a wide range of disciplines, and have come as the result of newly available genome sequences of pathogens such as candida albicans and other model fungi. In Candida Albicans: Methods and Prools, expert researchers explore these exciting new insights, focusing on the study of medically important fungi and Candida spp in particular. Chapters examine critical aspects of molecular methods, providing information on reporter gene assays, transformation, gene expression in vivo, and methods for large-scale gene disruption. At the same time, the work includes in-depth descriptions of disease models of candidiasis, facts about strain identification, and guidelines on the preparation of samples for proteomic investigations and tandem affinity purification. Composed in the highly successful "Methods in Molecular Biology™" series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting edge, Candida Albicans: Methods and Prools is an invaluable source of methods for investigators in the exhilarating fields of medical and molecular mycology.
Editorials
From The Critics
Reviewer: Rebecca T. Horvat, PhD, D(ABMM)(University of Kansas Medical Center)Description: This small book is directed specifically to researchers involved in the study of yeast associated with human disease. The topics are presented in six different sections that cover areas such as physiology and biochemistry as well as molecular biology and genome organization of these pathogens. Each chapter has procedures that would assist research laboratories in their approach to answers in this area.
Purpose: It is designed to provide detailed research protocols that will allow scientists to answer questions regarding how the Candida species can change from a normal flora to a pathogen. This would be a very useful resource to those laboratories focusing on the Candida species.
Audience: The authors anticipate that this book will be used by scientists and students in the field of fungal and yeast research. The authors of each chapter have sufficient experience as scientists to provide detailed information and tips on each procedure.
Features: The book starts with the immunologic studies that detect the host response to yeast infections. The methods describe such issues as detections of secretory IgA in body fluids and phagocytosis of the yeast by host immune cells. This section is followed by two sections on virulence and biofilms in which yeast behave differently than yeast growing on an artificial media. These chapters give advice on how to measure these differences. Chapters cover typing and identification as well as genetic methods. The last section, containing a single chapter, gives good advice on the use of growth media to grow the yeast for other studies. Each of the methods has step-by-step instructions with special hints for difficult issues. The book also provides alternative methods that can be used, along with references to these methods.
Assessment: This compact book, reasonably priced at less than $100, would clearly be a good investment for scientists studying or teaching about Candida species and virulence in humans. Another positive attribute is its small size, which makes it easy to carry around and fit on a lab bench.