Cardiovascular Proteomics: Methods and Protocols

From The Critics

Reviewer: Eugene A Davidson, PhD(Georgetown University School of Medicine)
Description: This book describes both basic and complex protocols used in studying the proteomics of the cardiovascular system.
Purpose: The goal is to offer a broad set of protocols for use in proteome analysis of cardiac tissue and related cells. The increasing use of proteomic analyses makes this timely and the effort is largely successful.
Audience: The audience for this book will include scientists working in the general area of atherosclerosis as well as those studying aspects of cardiac function. The editor has assembled a well qualified set of contributors.
Features: Investigators wish to have as much data as possible when studying complex systems, hence the advent of proteomics in virtually all areas of modern biology. This book provides a comprehensive survey of proteomic approaches to the study of cardiac function, related cell types, atherosclerosis, etc. The book is divided into six sections (31 chapters), each of which has protocols suitable for laboratory application. The initial material discusses common tools (two dimensional gels and mass spectrometry). This is followed by methods of cell fractionation (sub-proteome analysis as for mitochondria, for example), immunotechniques, and culture procedures for cardio-specific cell types. Concluding chapters have discussions of bioinformatics and phage display methods. Each chapter has a short bibliography to accompany detailed laboratory instructions. Overall, this is a comprehensive book that will be of interest to investigators working in the area of cardiac biology, atherosclerosis, and related topics.
Assessment: This is a thorough set of protocols for the cardiac biology community. Most laboratories will have some of these in place, but can profit from the broad coverage of this book.