Overview
Kinetic studies of enzyme action provide powerful insights into the underlying mechanisms of catalysis and regulation. These approaches are equally useful in examining the action of newly discovered enzymes and therapeutic agents.Contemporary Enzyme Kinetics and Mechanism, Second Edition presents key articles from Volumes 63, 64, 87, and 249 of Methods in Enzymology. The chapters describe the most essential and widely applied strategies. A set of exercises and problems is included to facilitate mastery of these topics.
The book will aid the reader to design, execute, and analyze kinetic experiments on enzymes. Its emphasis on enzyme inhibition will also make it attractive to pharmacologists and pharmaceutical chemists interested in rational drug design.
Of the seventeen chapters presented in this new edition, ten did not previously appear in the first edition.
Key Features
* Transient kinetic approaches to enzyme mechanisms
* Designing initial rate enzyme assay
* Deriving initial velocity and isotope exchange rate equations
* Plotting and statistical methods for analyzing rate data
* Cooperativity in enzyme function
* Reversible enzyme inhibitors as mechanistic probes
* Transition-state and multisubstrate inhibitors
* Affinity labeling to probe enzyme structure and function
* Mechanism-based enzyme inactivators
* Isotope exchange methods for elucidating enzymatic catalysis
* Kinetic isotope effects in enzyme catalysis
* Site-directed mutagenesis in studies of enzyme catalysis
The book contains black-and-white illustrations.
Synopsis
Kinetic studies of enzyme action provide powerful insights into the underlying mechanisms of catalysis and regulation. These approaches are equally useful in examining the action of newly discovered enzymes and therapeutic agents.
Contemporary Enzyme Kinetics and Mechanism, Second Edition presents key articles from Volumes 63, 64, 87, and 249 of Methods in Enzymology. The chapters describe the most essential and widely applied strategies. A set of exercises and problems is included to facilitate mastery of these topics.
Key Features
* Transient kinetic approaches to enzyme mechanisms
* Designing initial rate enzyme assay
* Deriving initial velocity and isotope exchange rate equations
* Plotting and statistical methods for analyzing rate data
* Cooperativity in enzyme function
* Reversible enzyme inhibitors as mechanistic probes
* Transition-state and multisubstrate inhibitors
* Affinity labeling to probe enzyme structure and function
* Mechanism-based enzyme inactivators
* Isotope exchange methods for elucidating enzymatic catalysis
* Kinetic isotope effects in enzyme catalysis
* Site-directed mutagenesis in studies of enzyme catalysis
Gene A. Homandberg
This multiauthored second edition monograph is, as was the first edition published in 1983, a compilation of basic articles from the Enzymes and Kinetics series of Methods in Enzymology. The chapters were written by 19 contributors from various specialties. Most of the chapters have been updated, and 10 of the 17 chapters are new. "This monograph is meant to update the earlier volume to continue to provide assistance in design, execution and analysis of enzyme systems. Since the first edition in 1983, the dramatic growth of this area has necessitated a newer edition. For example, this edition has been updated extensively and, for example, now includes a very interesting chapter on site-directed mutagenesis. "Although the audience is meant to be biochemists and biophysicists, this monograph is an ideal primary textbook for students in this area. "This softcover book features the typical attractive Methods in Enzymology format, with a suitable number of black-and-white illustrations. The book also features a chapter on selected exercises and problems of both a quantitative and descriptive nature. "This book is highly recommended because it contains basic information and discussion that covers earlier work to more recent work on enzyme dynamics. It is also very important because of the increasing need for more nonspecialists to be able to study newly discovered or expressed enzymes.
Editorials
From The Critics
Reviewer: Gene A. Homandberg, PhD(Rush Medical College of Rush University)Description: This multiauthored second edition monograph is, as was the first edition published in 1983, a compilation of basic articles from the Enzymes and Kinetics series of Methods in Enzymology. The chapters were written by 19 contributors from various specialties. Most of the chapters have been updated, and 10 of the 17 chapters are new.
Purpose: This monograph is meant to update the earlier volume to continue to provide assistance in design, execution and analysis of enzyme systems. Since the first edition in 1983, the dramatic growth of this area has necessitated a newer edition. For example, this edition has been updated extensively and, for example, now includes a very interesting chapter on site-directed mutagenesis.
Audience: Although the audience is meant to be biochemists and biophysicists, this monograph is an ideal primary textbook for students in this area.
Features: This softcover book features the typical attractive Methods in Enzymology format, with a suitable number of black-and-white illustrations. The book also features a chapter on selected exercises and problems of both a quantitative and descriptive nature.
Assessment: This book is highly recommended because it contains basic information and discussion that covers earlier work to more recent work on enzyme dynamics. It is also very important because of the increasing need for more nonspecialists to be able to study newly discovered or expressed enzymes.
Gene A. Homandberg
This multiauthored second edition monograph is, as was the first edition published in 1983, a compilation of basic articles from the Enzymes and Kinetics series of Methods in Enzymology. The chapters were written by 19 contributors from various specialties. Most of the chapters have been updated, and 10 of the 17 chapters are new. "This monograph is meant to update the earlier volume to continue to provide assistance in design, execution and analysis of enzyme systems. Since the first edition in 1983, the dramatic growth of this area has necessitated a newer edition. For example, this edition has been updated extensively and, for example, now includes a very interesting chapter on site-directed mutagenesis. "Although the audience is meant to be biochemists and biophysicists, this monograph is an ideal primary textbook for students in this area. "This softcover book features the typical attractive Methods in Enzymology format, with a suitable number of black-and-white illustrations. The book also features a chapter on selected exercises and problems of both a quantitative and descriptive nature. "This book is highly recommended because it contains basic information and discussion that covers earlier work to more recent work on enzyme dynamics. It is also very important because of the increasing need for more nonspecialists to be able to study newly discovered or expressed enzymes.Booknews
A collection of articles from vols. 63, 64, 87, and 249 of Methods in Enzymology, describing widely used strategies in kinetic studies of enzyme action and offering exercises and problems. Subjects include cooperativity in enzyme function, product inhibition applications, mechanism-based enzyme inactivators, and hydrogen tunneling in enzyme catalysis. Includes the complete table of contents for each of the volumes used in the collection. This second edition contains articles from vol. 249, and ten of the 17 chapters are new. Annotation c. Book News, Inc., Portland, OR (booknews.com)3 Stars from Doody