Cystic Fibrosis Methods and Protocols

Cystic Fibrosis: Methods and Prools consolidates cutting-edge in vitro, cellular, and whole animal laboratory prools into an indispensable resource. From electrophysiology and cell biology, to animal models and gene therapy, this comprehensive set of methods provides the step-by-step instructions needed for investigators to incorporate new approaches into their research programs. Specific prools describe new techniques for diagnosis, in vitro methods for the expression and functional analysis of CFTR, novel biochemical and cellular systems to determine how mutations subvert CFTR function, and in vivo prools to examine how CFTR dysfunction produce multisystem pathology in both human and animal models.

Since the cloning of the cystic fibrosis transmembrane conductance regulator (CFTR) nearly a decade ago, cystic fibrosis researchers, clinicians, and patients have come to rely increasingly on a diverse array of fundamental techniques to understand the molecular basis of this complex disease. Cystic Fibrosis Methods and Protocols consolidates a broad range of detailed and readily reproducible in vitro, cellular, and whole animal laboratory protocols into an indispensable resource. From electrophysiology and cell biology, to animal models and gene therapy, this comprehensive set of methods provides the step-by-step instructions needed for investigators to incorporate new approaches into their research programs. Specific protocols describe new techniques for diagnosis, in vitro methods for the expression and functional analysis of CFTR, novel biochemical and cellular systems to determine how mutations subvert CFTR function, and in vivo protocols to examine how CFTR dysfunction produces multisystem pathology in human and animal models.

Comprehensive, multidisciplinary, and highly practical, Cystic Fibrosis Methods and Protocols makes accessible to today's cystic fibrosis investigator the powerful new scientific techniques required to investigate the basic science of the disease and to translate this into effective clinical solutions.

Booknews

This handbook for medical researchers presents a wide array of in vitro, cellular, and whole animal protocols. Covering electrophysiology, cell biology, animal models, and gene therapy, the book provides step-by-step instructions for incorporating new approaches into research programs. Specific protocols describe new techniques for diagnosis, in vitro methods for the expression and functional analysis of CFTR, novel biochemical and cellular systems for determining the role of mutations, and in vivo protocols to examine how CFTR dysfunction produces multisystem pathology. The book emphasizes the translation of recent scientific advances into clinical solutions. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Skach, William R., MD