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Overview
With the completion of the mouse and human genome sequences, the functional characterization of every mammalian gene and the deciphering of their molecular interaction network has become the next major challenge, and the mouse genome provides a convenient and vital model for these studies. In "Gene Knockout Prools, Second Edition", distinguished contributors with extensive experience in the gene targeting and mouse genetics fields reveal a comprehensive collection of step-by-step laboratory prools. Emphasizing the many new mutagenesis techniques developed over the last seven years, the volume covers topics such as gene modification in ES cells, stem cell manipulation, the generation of genetically engineered mice, and mutant phenotype analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, readily reproducible prools, and notes on troubleshooting and avoiding known pitfalls.
Fully updated and authoritative, "Gene Knockout Prools, Second Edition" provides a unique collection of expert bench prools certain to be a valuable resource for those just entering the field of gene targeting but also for all genetic researchers today.
Synopsis
With the completion of the mouse and human genome sequences, the functional characterization of every mammalian gene and the deciphering of their molecular interaction network has become the next major challenge, and the mouse genome provides a convenient and vital model for these studies. In "Gene Knockout Protocols, Second Edition", distinguished contributors with extensive experience in the gene targeting and mouse genetics fields reveal a comprehensive collection of step-by-step laboratory protocols. Emphasizing the many new mutagenesis techniques developed over the last seven years, the volume covers topics such as gene modification in ES cells, stem cell manipulation, the generation of genetically engineered mice, and mutant phenotype analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Fully updated and authoritative, "Gene Knockout Protocols, Second Edition" provides a unique collection of expert bench protocols certain to be a valuable resource for those just entering the field of gene targeting but also for all genetic researchers today.
Doody Review Services
Reviewer:Omer Iqbal, MD, FACC, FESC(Loyola University Medical Center)
Description:This comprehensive collection of step-by-step protocols in the field of gene targeting and mouse genetics covers such topics as gene modification in embryonic stem cells, stem cell manipulation, the generation of genetically engineered mice, and mutant phenotype analysis.
Purpose:The purpose is to provide protocols for both beginners and experienced scientists in the field.
Audience:The book will serve as a valuable resource for researchers, scientists, and students interested in these areas.
Features:Following an overview of mouse mutagenesis, part I deals with gene modification in embryonic stem cells. Part II then covers stem cell manipulation, part III explores genetically engineered mice, and part IV deals with phenotype analysis. The book is illustrated with numerous black-and-white photographs and figures and a number of colored plates.
Assessment:This is a well-illustrated book written by distinguished experts in the fields of gene targeting and mouse genetics. All researchers interested in gene targeting and gene knockout protocols will find this extremely useful. Given the significant advances in this field, the second edition is justified.
Editorials
From The Critics
Reviewer: Omer Iqbal, MD, FACC, FESC(Loyola University Medical Center)Description: This comprehensive collection of step-by-step protocols in the field of gene targeting and mouse genetics covers such topics as gene modification in embryonic stem cells, stem cell manipulation, the generation of genetically engineered mice, and mutant phenotype analysis.
Purpose: The purpose is to provide protocols for both beginners and experienced scientists in the field.
Audience: The book will serve as a valuable resource for researchers, scientists, and students interested in these areas.
Features: Following an overview of mouse mutagenesis, part I deals with gene modification in embryonic stem cells. Part II then covers stem cell manipulation, part III explores genetically engineered mice, and part IV deals with phenotype analysis. The book is illustrated with numerous black-and-white photographs and figures and a number of colored plates.
Assessment: This is a well-illustrated book written by distinguished experts in the fields of gene targeting and mouse genetics. All researchers interested in gene targeting and gene knockout protocols will find this extremely useful. Given the significant advances in this field, the second edition is justified.