Biology - Molecular Biology, Medical Research, Analytical Chemistry - Chromatography
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Overview
This important contribution to the scientific community explains various aspects of reverse-phase separations. How to Use Reverse-Phase HPLC surveys the basics of liquid chromatography and summarizes the theoretical aspects of reverse-phase HPLC. Chapters also discuss the influence of stationary and mobile phases on the efficiency and selectivity of the separations, the use of conventionally used and special reverse phase packing as well as that of masking agents added in the mobile phase, the evaluation of column performance in reverse phase chromatography, the applicability of special methods and techniques in RP-HPLC, the most important practical aspects of phase system optimization, and HPLC method validation summarizing the practical approaches recommended for the design and performance of validation experiments.Editorials
Booknews
A self-teaching text that explains the state-of-the-art capabilities of High Performance Liquid Chromatography (HPLC) with a heavy emphasis on the more versatile reverse phase mode. Those unfamiliar with HPLC receive a review of the basics and a discussion of the theory behind the technology, as well as a hands-on approach to learning the techniques and what they can do for their research. The text emphasizes the specific interaction between solutes and stationary/mobile phases and the influence of stationary and mobile phases on the efficiency and selectivity of separations. Includes a discussion of validation experiments. Annotation c. Book News, Inc., Portland, OR (booknews.com)Book Details
Published
April 1, 1992
Publisher
Wiley, John & Sons, Incorporated
Pages
356
Format
Hardcover
ISBN
9780895737663