Books.org participates in affiliate programs including Bookshop.org and the Amazon Services LLC Associates Program. We may earn a commission from qualifying purchases made through links on this page, at no additional cost to you.
Overview
Human Genome Methods is a practical guide to the application of molecular biology and genetics techniques to research on human cells. Written by recognized authorities who often originated the techniques described, chapters present experimental protocols that are readily used at the laboratory bench. The step-by-step protocols are concise and easy to follow to be reproducible by researchers of various levels of expertise. Suggestions for successful application of procedures are included, along with recommended materials and suppliers. Helpful background information and results of applying the methods described are also given.
Section I covers topics such as microsatellite DNA, dynamic mutations, gene targeting using the DNA triple helix, and protease footprinting of DNA-protein interactions. This is followed in Section II by discussions of in situ hybridization, cell synchronization, and cell cycle specific gene expression. Methods concerned with programmed cell death are explored in Section III, which covers this emerging research area and the culture and analysis of cancer cells. Section IV presents methods related to transgene analysis of mouse embryonic stem cells, generation and knockout studies with null mutant mice, and mouse models for human disease. The final section reviews genome mapping, with an emphasis on the construction of linkage maps and on somatic cell hybrids for mapping disease genes.
The book contains black-and-white illustrations.
Editorials
From The Critics
Reviewer: Michael Cummings, PhD(University of Illinois at Chicago)Description: In human genetics, the in vitro growth of somatic cells has played an important role in dissecting gene action and in gene mapping. The development and widespread use of cell culture has generated a range of books containing methods and applications for use on cells in culture.
Purpose: This book is intended to be a practical guide for the use of molecular techniques on human cells.
Audience: It is written for geneticists, molecular biologists, and graduate students. The text is divided into five sections covering DNA analysis; gene expression; programmed cell death: cell culture; transgenes, knockout mice, and mouse models; and genome mapping.
Features: Unfortunately, the book fails at several levels in its goals to be a practical guide with experimental protocols to be used at the lab bench. Not all the chapters deal with cultured human cells, even granting the stretch to include mouse stem cells and the generation of chimeric mice. The chapter on genome mapping is a review of general methods used in mapping human genes and ends with a list of databases containing maps. The chapter does not mention cell culture, nor does it present any methods that could be used at the lab bench. Most of the other chapters are more like short review articles with only general descriptions of methods. Where methods are presented, they are not organized in a step-by-step format, but are embedded in the text, making them difficult to follow when used at the lab bench.
Assessment: There are many better books on the use of molecular techniques in cell culture, and it is difficult to recommend this book to beginners or experienced investigators.
Michael Cummings
In human genetics, the in vitro growth of somatic cells has played an important role in dissecting gene action and in gene mapping. The development and widespread use of cell culture has generated a range of books containing methods and applications for use on cells in culture. This book is intended to be a practical guide for the use of molecular techniques on human cells. It is written for geneticists, molecular biologists, and graduate students. The text is divided into five sections covering DNA analysis; gene expression; programmed cell death: cell culture; transgenes, knockout mice, and mouse models; and genome mapping. Unfortunately, the book fails at several levels in its goals to be a practical guide with experimental protocols to be used at the lab bench. Not all the chapters deal with cultured human cells, even granting the stretch to include mouse stem cells and the generation of chimeric mice. The chapter on genome mapping is a review of general methods used in mapping human genes and ends with a list of databases containing maps. The chapter does not mention cell culture, nor does it present any methods that could be used at the lab bench. Most of the other chapters are more like short review articles with only general descriptions of methods. Where methods are presented, they are not organized in a step-by-step format, but are embedded in the text, making them difficult to follow when used at the lab bench. There are many better books on the use of molecular techniques in cell culture, and it is difficult to recommend this book to beginners or experienced investigators.1 Star from Doody