Viral Gene Techniques

H. Steven Seifert

This is the sixth volume in a series of specialized technique books edited by Kenneth Adolph. Each chapter of this book describes detailed methods for conducting molecular biological investigations into eukaryotic, prokaryotic, and bacteriophage microbes. The purpose is to provide detailed experimental techniques of systems that are not usually found in the standard molecular biology laboratory manuals. It is targeted for laboratory personnel of all levels. Although most of the chapters describe a specific organism, the techniques are applicable to a wide range of organisms or systems. The book covers the following organisms and/or techniques: genetics of Toxoplasma gondii, protozoan genome analysis, Aspergillus nidulans, molecular techniques for filamentous fungi, yeast chromosome fragmentation and artificial chromosomes, transcription factor binding to nucleosomes in yeast, yeast nucleosome mapping, assays for ligation and cleavage of site-specific recombinases, physical analysis of yeast recombination, genetic analysis of yeast DNA-dependent RNA polymerases, pheromones in fission yeast, use of methylation deficient E. coli, RNA end analysis in bacteria, E. coli capsule gene regulation, genetic mapping in B. subtilis, chemotaxis in B.subtilis, cloning of Thermomonospora fusca genes, computational analyses, in vitro transcription and phage lambda, protein-protein interactions in the replication of phage P1, transcription in B. subtilis phage phi 29, use of coliphages for genetic analysis of Erwinia, and assembly of bacteriophage PRD1 and phi 6 particles. This book contains many very detailed methods that will not only beuseful for the organisms described, but will also be adaptable to other systems. Most of the chapters are well written and provide sufficient detail to assist either a neophyte or a seasoned veteran. This fills a need for detailed techniques and methods that lie outside of the areas of concentration found in the standard manuals.

From The Critics

Reviewer:H. Steven Seifert, PhD(Northwestern University Feinberg School of Medicine)
Description:This is the sixth volume in a series of specialized technique books edited by Kenneth Adolph. Each chapter of this book describes detailed methods for conducting molecular biological investigations into eukaryotic, prokaryotic, and bacteriophage microbes.
Purpose:The purpose is to provide detailed experimental techniques of systems that are not usually found in the standard molecular biology laboratory manuals.
Audience:It is targeted for laboratory personnel of all levels. Although most of the chapters describe a specific organism, the techniques are applicable to a wide range of organisms or systems.
Features:The book covers the following organisms and/or techniques: genetics of Toxoplasma gondii, protozoan genome analysis, Aspergillus nidulans, molecular techniques for filamentous fungi, yeast chromosome fragmentation and artificial chromosomes, transcription factor binding to nucleosomes in yeast, yeast nucleosome mapping, assays for ligation and cleavage of site-specific recombinases, physical analysis of yeast recombination, genetic analysis of yeast DNA-dependent RNA polymerases, pheromones in fission yeast, use of methylation deficient E. coli, RNA end analysis in bacteria, E. coli capsule gene regulation, genetic mapping in B. subtilis, chemotaxis in B.subtilis, cloning of Thermomonospora fusca genes, computational analyses, in vitro transcription and phage lambda, protein-protein interactions in the replication of phage P1, transcription in B. subtilis phagephi 29, use of coliphages for genetic analysis of Erwinia, and assembly of bacteriophage PRD1 and phi 6 particles.
Assessment:This book contains many very detailed methods that will not only be useful for the organisms described, but will also be adaptable to other systems. Most of the chapters are well written and provide sufficient detail to assist either a neophyte or a seasoned veteran. This fills a need for detailed techniques and methods that lie outside of the areas of concentration found in the standard manuals.

3 Stars from Doody