Microscopes & Microscopy - Electron Microscopes, Biochemistry - General & Miscellaneous

Hybridization Techniques for Electron Microscopy

Name: Hybridization Techniques for Electron Microscopy
Author: Gerard Morel
ISBN: 9780849344145

by Gerard Morel

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Overview

Hybridization Techniques for Electron Microscopy examines the use of in situ hybridization techniques, including an overview of current perspectives and future developments. The book features in situ methods for fluorescence probes and confocal scanning microscopes. Three in situ hybridization methods for electron microscopes are analyzed: the non-embedded tissue method using ultrathin frozen sections, pre-embedded method, and post-embedded method using material embedded in hydrophilic resin. Positive and negative features are discussed, and clear instructions regarding implementation of techniques are provided.

Particular aspects of the techniques are examined in detail, such as preparation of tissue, pretreatment, hybridization procedures, revelation (autoradiography and immunocytology) and checking procedures, in addition to the illustration, interpretation, and discussion of methods and results. The main applications described include virus detection, chromosomal gene mapping, detection of ribosomic nucleic acid, and detection of messenger RNA in animals and plants. Hybridization Techniques for Electron Microscopy is an excellent reference for cytologists, cell biologists, histochemists, cytochemists, molecular endocrinologists, and neuroendocrinologists.

This book contains black-and-white illustrations.

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Editorials

From The Critics

Reviewer: Alvin Telser, PhD(Northwestern University Feinberg School of Medicine)
Description: This is a new book presenting detailed nucleic acid hybridization techniques for electron microscopy.
Purpose: It is a "how-to-do" version of a graduate course taught by the author for the past several years. Although the techniques are quite useful and important, techniques such as these often change so quickly that a book like this is outdated within a year or two of publication. The book appears to meet the author's expectations.
Audience: The primary audience is researchers (faculty, postdoctoral fellows, and/or graduate students) who are unfamiliar with these techniques. The techniques can be quite difficult to execute reliably; because this book is so detailed, it may be of value to someone having technical difficulties. Each chapter is written by different contributors, and the volume is edited by Professor Morel.
Features: The book contains many drawings, diagrams, and electron micrographs illustrating how to do the techniques and what results may be expected. The book has a good table of contents and index.
Assessment: This is a rather expensive book that is attractive and well written (given that most of the text describes techniques). There is a reasonable likelihood that the book will be outdated in the next year or two. It might be better used in the laboratory as a cookbook of how to actually do the procedures than used as a reference book in a library. The book title is somewhat misleading, because a reader may get the impression that more than nucleic acid hybridization techniques are covered.

Alvin Telser

This is a new book presenting detailed nucleic acid hybridization techniques for electron microscopy. It is a ""how-to-do"" version of a graduate course taught by the author for the past several years. Although the techniques are quite useful and important, techniques such as these often change so quickly that a book like this is outdated within a year or two of publication. The book appears to meet the author's expectations. The primary audience is researchers (faculty, postdoctoral fellows, and/or graduate students) who are unfamiliar with these techniques. The techniques can be quite difficult to execute reliably; because this book is so detailed, it may be of value to someone having technical difficulties. Each chapter is written by different contributors, and the volume is edited by Professor Morel. The book contains many drawings, diagrams, and electron micrographs illustrating how to do the techniques and what results may be expected. The book has a good table of contents and index. This is a rather expensive book that is attractive and well written (given that most of the text describes techniques). There is a reasonable likelihood that the book will be outdated in the next year or two. It might be better used in the laboratory as a cookbook of how to actually do the procedures than used as a reference book in a library. The book title is somewhat misleading, because a reader may get the impression that more than nucleic acid hybridization techniques are covered.

2 Stars from Doody