Overview
This book is a practical guide to the preparation and use of immobilized affinity ligands for purification, catalysis, and analysis. Special emphasis is given to immunochemical techniques including antibody isolation, preparation of antibody fragments using immobilized enzymes, and immunoaffinity chromatography. The book provides easy-to-follow, well-tested protocols to allow the uninitiated to use these techniques to the maximum advantage with minimum hassle. In addition, it shows researchers how to save money by making their own optimized affinity supports.Key Features
* Matrix activation
* Ligand immobilization
* Binding and elution of target molecules
* Enzyme catalysis on solid supports
* Analytical affinity chromatography
* Isolation/purification of antibodies
* Preparation of antibody fragments
* Immunoaffinity chromatography
* Immobilization of nucleic acids
* Use of immobilized ligands for removal of trace contaminants Practical advice on choosing:
* Matrices
* Spacers
* Methods of activation and coupling Background information and insights on:
* Affinity interactions
* The ease and power of affinity chromatography
* Attaching molecules to insoluble supports
* Matrices currently in use
* Over 20 methods of activation
* Spacers
* Extensive References
Audience: Employees of biotechnology companies, graduate students and researchers in biochemistry, molecular biology, immunology, pharmacology, cell biology, microbiology, enzymology, veterinary science, and clinical chemistry.