Matrix Metalloproteinase Protocols, Vol. 622

Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode worms. Fully updating and adding to the popular first edition, Matrix Metalloproteinase Prools, Second Edition includes a series of state-of-the-art techniques provided by eminent experts in the field. Beginning with a brief overview of the MMP arena, from how these enzymes fit into the larger degradome to what occurs when their expression and function in the mouse is modulated, the volume continues with sections on the expression and purification of MMPs and TIMPs, the detection of MMPs and TIMPs at both the protein and mRNA level, and our ability to assay MMP and TIMP activities in a wide variety of circumstances. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Matrix Metalloproteinase Prools, Second Edition is an ideal source for many of the essential laboratory techniques for both novice and seasoned researchers alike collected in one convenient volume.

The book contains black-and-white illustrations.

This is the first comprehensive collection of protocols specific for this field. Protocols that are important in research on a growing family of proteolytic enzymes, the matrix metalloproteinases (MMPs) and the tissue inhibitors of metalloproteinases (TIMPs) are reviewed. Much of the methodology appropriate for the detection and assay of these proteins is covered, as well as their expression and purification. MMPs and TIMPs are implicated in many physiological and pathological situations where the extracellular matrix is turned over or degraded, so that they represent important therapeutic targets in a number of diseases. This book will enable laboratories to have a wide range of relevant techniques right at their fingertips, while at the same time provide novice researchers with a starting point for their work and broaden the methods available to established groups. The wealth of knowledge and experience provided are invaluable, since they provide working methodologies that have been perfected over time from some of the best MMP labs in the world. For this reason they represent important therapeutic targets in a number of diseases. This book provides laboratories with a wide range of relevant techniques to serve as a springboard for their work and broaden the established repertoires of experienced researchers.

Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode worms. Fully updating and adding to the popular first edition, Matrix Metalloproteinase Protocols, Second Edition includes a series of state-of-the-art techniques provided by eminent experts in the field. Beginning with a brief overview of the MMP arena, from how these enzymes fit into the larger degradome to what occurs when their expression and function in the mouse is modulated, the volume continues with sections on the expression and purification of MMPs and TIMPs, the detection of MMPs and TIMPs at both the protein and mRNA level, and our ability to assay MMP and TIMP activities in a wide variety of circumstances. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Matrix Metalloproteinase Protocols, Second Edition is an ideal source for many of the essential laboratory techniques for both novice and seasoned researchers alike collected in one convenient volume.

Booknews

Details proven techniques for expression and purification of matrix metalloproteinases (MMPs) and TIMPs, and for the detection of MMPs and TIMPs at both protein and mRNA levels. Part I overviews the MMP arena, and Parts II and III present methods with step-by-step instructions and notes on variant applications and pitfalls. Part IV shows how to assay MMP and TIMP activities in a wide variety of circumstances. Of interest to those in chemistry, biology, and histology. Specific topics include strategies for cloning new MMPs and TIMPs, expression of recombinant membrane-type MMPs, and antibodies to MMP-cleaved aggrecan. Clark is affiliated with the University of East Anglia. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Clark, Ian M., PhD (Univ of East Anglia, Norwich)