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Overview
While the theoretical basis of centrifugal separations remains the same, recent years have seen dramatic changes in both the design of centrifuges and the range of people that use them. This book reflects these changing uses of preparative centrifuges by providing detailed protocols covering all of the different types of separation from DNA to cells. Guidelines are also given to help the reader devise new types of separation protocols. The book includes program source code for calculation and simulation programs that are invaluable for quantitative centrifugation methods. In addition, this volume contains extensive appendices of valuable data that are required by everyone using centrifuges as part of their research work. Preparative Centrifugation: A Practical Approach contains a wealth of guidelines, protocols, and practical advice that will be of direct use to experienced researchers and novices alike in virtually every area of biological research.
This book contains black-and-white illustrations.
Editorials
From The Critics
Reviewer: Earle W. Holmes, PhD(Loyola University Medical Center)Description: This book presents the theoretical and practical aspects of the use of centrifugation in the purification and characterization of cells, subcellular fractions, and macromolecules.
Purpose: The purpose is to provide detailed information on specific techniques that can be immediately applied in the reader's laboratory, but at the same time, to provide enough general information to assist in the development of new applications. Both goals have been met.
Audience: The book should be a valuable reference for graduate students in the biological sciences and for investigators who intend to apply centrifugation techniques to their research.
Features: This is an authoritative reference because the author and the contributors have many years of experience in the development and use of the techniques about which they write. Particularly valuable are the numerous appendixes containing computer programs for the determination of rotor parameters and sedimentation coefficients, tables listing the properties of density gradient solutions, and procedures for assaying marker enzymes of subcellular fractions.
Assessment: This is an excellent source of practical knowledge in the application of centrifugation to the biological sciences. Although some of the subject matter could probably be found in other publications to which the authors have previously contributed, this book contains an impressive amount of essential and useful information. It will be a popular reference book in the library of any institution where biological research is carried out, and it will also have a place on the bookshelves of students, teachers, o r investigators who wish to have a single comprehensive reference on the subject.
3 Stars from Doody